Objective：To establish a chronic stress mouse model，and observe the regulatory effect of Shichangpu volatile oil microemulsion on the memory of mice model，so as to discuss its mechanism preliminarily.Methods：Divided 36 Kunming(KM)mice into 3 groups randomly， 12 cases in each group， namely blank control group， chronic stress group and combination of chronic stress and Shichangpu volatile oil microemulsion group(the treatment group).Blank control group receivedthe normal diet，chronic stress group received “the chronic restraint stress experiment”，and the treatment group received the intragastric administration with 4.5 g/(kg·d)of microemulsion of Shichangpu volatile oil as well as“the chronic restraint stress experiment”.Observed the general state of mice，and measured their body mass.Observed the spatial learning and memory ability of mice by means of Morris water maze，and detected the level of serum glucocorticoid.Results：At the 28th day of the experiment， comparing with blank control group， body mass of the chronic stress group was decreased evidently，the difference being significant(P＜0.05).Comparing with chronic stress group，body mass of the treatment group was increased evidently，the difference being significant(P＜0.05).Comparing with blank control group，the latencies of mice in chronic stress group were evidently longer，times of passing through the platform were also reduced significantly，and levels of plasma cortisol were relatively high，differences being significant(P＜0.01).Comparing with the chronic stress group，the latencies of mice in the treatment group were shortened，times of passing through the platform were also increased， and levels of plasma cortisol were declined significantly，differences being significant(P＜0.01).Conclusion：The microemulsion of Shichangpu volatile oil has the function of improving the memory of mice with chronic stress，the mechanism of which is related to the decrease of levels of serum glucocorticoid.