HPLC法对决明子中3种有效成分含量的测定
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R284.1

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Determination of Content of Three Effective Components in Cassia Seed with HPLC
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    摘要:

    目的:高效液相色谱法测定决明子中红镰霉素龙胆二糖苷、决明子苷C及橙黄决明素-6-O-β-D-葡萄糖苷3种有效成分的含量。方法:采用Zorbax Eclipse XDB-C18色谱柱,柱温30℃,以乙腈和四氢呋喃混合溶液(A)-1%冰醋酸(B)(A∶B=30∶70)为流动相等度洗脱,流速1.0 mL/min,检测波长为278 nm。结果:3种有效成分得到了基线分离。红镰霉素龙胆二糖苷、决明子苷 C 及橙黄决明素-6-O-β-D-葡萄糖苷分别在 0.136 4~1.091 μg、0.117 9~0.943 μg、0.126 3~1.010 μg 与相应的峰面积呈现良好的线性关系,回归方程分别为Y=3.78×106X-143 929、Y=2.67×106X-78 435、Y=3.15×106X-108 435;平均加样回收率分别为100.53%、98.95%、102.30%。对不同产地决明子药材进行测定,结果显示3种有效成分的含量差异较大。结论:经过方法学验证,本方法准确、可靠、重复性好,可以为决明子药材评价与质量控制提供有效参考。

    Abstract:

    Objective:To establish methods of high performance liquid chromatograph(HPLC)so as to determine content of rubrofusarin gentiobioside,assiaside C and Aurantio-obtusifolin-6-O-β-D-glucopyranoside of cassia seed.Methods:Performed Zorbax Eclipse XDB-C18 column at the temperature of 30℃,and used acetonitrile and tetrahydrofuran(A)-1%acetic acid(B)(A∶B=30∶70)as mobile phase by isocratic elution,with the flow rate being 1.0 mL/min and detection wavelength at 278 nm.Results:Three effective components achieved a baseline resolution.Rubrofusarin gentiobioside,assiaside C and Aurantio-obtusifolin-6-O-β-D-glucopyranoside respectively demonstrated a good linear relationship with the peak area in the range of 0.136 4~1.091 μg,0.117 9~0.943 μg,and 0.126 3~1.010 μg.The respective typical equation was Y=3.78×106X-143 92,Y=2.67×106X-784 35,Y=3.15×106X-108 435 respectively,and their average recovery was 100.53%,98.95%and 102.30% respectively.For the determination of cassia seed from different habitats,the result indicated there was great difference among the content of these three effective components.Conclusion:By methodologyverification,the determination method is accurate,reliable,and of good reproducibility.It can be an effective reference to the evaluation and quality control of cassia seed.

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张梅,冯良,潘娜. HPLC法对决明子中3种有效成分含量的测定[J].新中医,2018,50(1):15-18

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  • 在线发布日期: 2018-01-05
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