不同剂量小青龙汤变方对发作期哮H器小鼠体内NO含量及NOS影响的研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

R256.12

基金项目:


Modified Prescription of Xiaoqinglong Tang in Different Doses Has effect on NO Content and NOS in Asthmatic Mice at the Attack Stage
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:观察不同剂量小青龙汤变方对发作期哮喘小鼠体内一氧化氮(Nitric Oxide,NO)含量及一氧化氮合酶(Nitric Oxide Synthase,NOS)的影响,探讨该方在哮喘急性期可能的作用机制。方法:将BALB/c小鼠按随机数字表法分为空白组、模型组、小青龙汤变方低剂量组、小青龙汤变方高剂量组、激素组,每组10只。采用注射卵白蛋白和氢氧化铝凝胶混悬液的方法制作哮喘小鼠模型,用不同剂量小青龙汤变方及地塞米松进行干预治疗后,测定各组小鼠血清、肺组织匀浆液中NO、诱导型一氧化氮合酶(inducible Nitric Oxide Synthase,iNOS)、构成型一氧化氮合酶(constitutive Nitric Oxide Synthase,cNOS)活性的变化水平,探讨所检测指标与急性期哮喘的相关性;并通过肺组织病理学检查观察各组肺组织炎性细胞的浸润情况。结果:与空白组比较,模型组小鼠体内血清及肺组织匀浆中NO及iNOS含量明显增高(P<0.05),cNOS变化不明显,肺组织病变严重。与模型组比较,药物干预后,小青龙汤变方低、高剂量组及激素组小鼠体内NO及iNOS含量均下降,以小青龙汤变方高剂量组及激素组降低最明显,差异有统计学意义(P<0.05)。肺组织病理切片显示激素组、小青龙汤变方低、高剂量组较模型组炎性改变有明显好转,以激素组和小青龙汤变方高剂量组病变最为轻微。结论:小青龙汤变方可降低哮喘发作期小鼠体内iNOS含量、抑制其活性表达,降低NO含量,从而改善哮喘症状及肺组织病理变化,且在一定范围内其药效作用随着药量的增加而增加,可见中医药复方在治疗疾病中多途径、多靶点的疗效优势。

    Abstract:

    Objective:To observe the effect of modified prescription of Xiaoqinglong tang in different doses on nitric oxide(NO)content and nitric oxide synthase(NOS)in asthmatic mice at the attack stage,and to discuss the possible functioning mechanism of the prescription at acute attack of asthma.Methods:Divided BALB/c mice into the blank group,the model group, the low-dose and high-dose modified prescription of Xiaoqinglong tang group and the hormone group randomly,10 mice in each group.Injection with suspension of ovalbumin and aluminum hydroxide gel was administered to establish the model of asthmatic mice.After using the modified prescription of Xiaoqinglong tang in different doses and dexamethasone to intervene the treatment,measured the changes of activity of NO,iNOS and cNOS in serum and lung tissue homogenate of mice in each goup, discussed the relevance between the indexes detected above and asthma in acute attack;and by means of examining pathological examination of lung tissue,observed the infiltration of inflammatory cells of lung tissue in each group.Results:Comparing with the blank group,the content of NO and iNOS in serum and lung tissue homogenate of mice in the model group was significantly increased(P<0.05),cNOS was not significantly changed,and the lesions of lung tissue were severe.Comparing with the model group,the content of NO and iNOS of mice in the low-dose and high-dose modified prescription of Xiaoqinglong tang group as well as the hormone group was respectively decreased(P<0.05),and the high-dose modified prescription of Xiaoqinglong tang group and the hormone group were decreased the most significantly;the pathological section of lung tissue showed that the inflammatory lesion of the hormone group and the low-dose and high-dose modified prescription of Xiaoqinglong tang group was significantly improved,and the lesion of the hormone group and the high-dose modified prescription of Xiaoqinglong tang group was the slightest.Comparing with the low-dose Chinese medicine group, the content of NO in serum and lung tissue homogenate, the content of TNOS and iNOS in serum and the content of TNOS and iNOS in lung tissue homogenate of mice in the high-dose group were significantly decreased,differences being significant(P<0.05).Conclusion:The modified prescription of Xiaoqinglong tang can reduce iNOS content and inhibit its activity expression in mice at the attack stage of asthma and decrease the excessive production of NO,thus to improve asthmatic symptoms and the pathological changes of lung tissue,and its efficacy enhances as the dosage increases in certain scope,which shows multiple-approach and multi-target as the clinical advantage of Chinese herbal compound in the treatment of disease.

    参考文献
    相似文献
    引证文献
引用本文

徐瑞,张晓.不同剂量小青龙汤变方对发作期哮H器小鼠体内NO含量及NOS影响的研究[J].新中医,2018,50(5):31-35

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2018-05-03
  • 出版日期: