丹参提取物对系膜增生性肾小球肾炎大鼠TGF-β1/p38MAPK信号通路的影响研究
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Extract of Radix et Rhizoma Salviae Miltiorrhizae has Effect on TGF-β1/p38MAPK Signaling Pathways in Rats with Mesangial Proliferative Glomerulonephritis
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    摘要:

    目的:观察丹参提取物对系膜增生性肾小球肾炎(Mesangial proliferative glomerulonephritis,MsPGN)大鼠肾组织转化因子-β1(Transforming growth factor-β1,TGF-β1) 和 p38丝裂原活化蛋白激酶 (p38 mitogen-activated protein kinase,p38MAPK)的影响。方法:将46只SD大鼠分为正常组、缬沙坦组、模型组、丹参提取物组,除正常组外,其余各组大鼠建立MsPGN模型。丹参提取物组按丹参5 g/(kg·d)的剂量灌胃给药;缬沙坦组按缬沙坦0.01 g/(kg·d)的剂量溶于温水灌胃,模型组和正常组每天给予等量温水灌胃,连续灌胃12周后处死大鼠。Western blot检测大鼠肾组织TGF-β1和p38 MAPK蛋白的表达,RT-PCR检测TGF-β1和p38MAPK的mRNA表达。结果:与正常组比较,模型组大鼠24 h尿蛋白水平、TGF-β1和p38MAPK蛋白相对表达量及mRNA表达均显著升高(P<0.05);与模型组比较,给药6周和12周缬沙坦组、丹参提取物组24 h尿蛋白水平显著降低(P<0.05);TGF-β1和p38MAPK蛋白相对表达量及mRNA表达均显著下降(P<0.05);与缬沙坦组比较,丹参提取物组TGF-β1和p38MAPK mRNA表达均下降(P<0.05)。结论:丹参提取物的肾保护作用可能是通过下调TGF-β1、p38MAPK蛋白和mRNA水平,抑制TGF-β1/p38MAPK信号通路激活来实现的。

    Abstract:

    Objective:To observe the effect of extract of Radix et Rhizoma Salviae Miltiorrhizae on transforming growth factor-β1(TGF-β1)and p38 mitogen-activated protein kinase(p38MAPK)in rats with mesangial proliferative glomerulonephritis(MsPGN).Methods:A total of 46 SD rats were divided into the normal group,the valsartan group,the model group and the extract of Radix et Rhizoma Salviae Miltiorrhizae group.The rat models with MsPGN were established in all groups except the normal group.The extract of Radix et Rhizoma Salviae Miltiorrhizae group was given 5 g/(kg·d)of Radix et Rhizoma Salviae Miltiorrhizae by gavage;the valsartan group was given 0.01 g/(kg·d)of valsartan dissolved in warm water by gavage,and the model group and the normal group were daily given the same amount of warm water by gavage.After 12-week gavage,all rats were sacrificed.The protein expression of TGF-β1 and p38MAPK in the kidney tissue of rats was detected by Western blot,and the mRNA expression of TGF- β1 and p38MAPK was detected by RT-PCR.Results:Compared with those in the normal group,the level of 24-hour urine protein and the relative protein expression as well as the mRNA expression of TGF-β1 and p38MAPK in rats in the model group were significantly increased(P < 0.05);compared with that in the model group,the levels of 24-hour urine protein in the valsartan group and the extract of Radix et Rhizoma Salviae Miltiorrhizae group after medication for six weeks and 12 weeks respectively were significantly decreased(P< 0.05);the relative protein expression as well as the mRNA expression of TGF-β1 and p38MAPK in the valsartan group and the extract of Radix et Rhizoma Salviae Miltiorrhizae group were significantly decreased(P < 0.05);compared with that in the valsartan group,the mRNA expression of TGF-β1 and p38MAPK in the extract of Radix et Rhizoma Salviae Miltiorrhizae group was decreased(P< 0.05).Conclusion:The renal protection of extract of Radix et Rhizoma Salviae Miltiorrhizae may be achieved by down-regulating the levels of protein and mRNA of TGF-β1 and p38MAPKand inhibiting the activation of TGF-β1/p38MAPKsignaling pathways.

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徐文燕,管青聪,徐芳.丹参提取物对系膜增生性肾小球肾炎大鼠TGF-β1/p38MAPK信号通路的影响研究[J].新中医,2020,52(2):5-9

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  • 在线发布日期: 2020-01-14
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