Abstract： Objective： To investigate the effect of Jianpi Yiwei prescription on serum gastrin， plasma motilin and inflammatory factors in patients with chronic atrophic gastritis(CAG) with spleen- stomach deficiency cold syndrome. Methods：A total of 82 cases of CAG patients with spleen-stomach deficiency cold syndrome were divided into two groups according to the random number table method，41 cases in each group. The control group was given the oral administration of amoxycillin capsules，omeprazole enteric-coated capsules and clarithromycin dispersible tablets；the observation group was given Jianpi Yiwei prescription. Both groups were treated for eight weeks. The curative effect was observed. Before and after intervention，the changes in the levels of serum gastrin，plasma motilin as well as interleukin- 6(IL- 6) and tumor necrosis factor- α(TNF- α) in serum of the two groups were compared. Results：After intervention，the levels of serum gastrin and plasma motilin in the observation group were increased and higher than those in the control group(P＜0.05)，and the levels of TNF-α and IL-6 in serum of the observation group were decreased and lower than those in the control group(P＜0.05). After intervention，the total effective rate was 92.68% in the observation group，higher than that of 75.61% in the control group (χ2=4.479，P=0.034＜0.05). In the control group，there were two cases of headache and two cases of diarrhea. Conclusion： The application of Jianpi Yiwei prescription for CAG patients with spleen-stomach deficiency cold syndrome can improve the secretion of serum gastrin and plasma motilin，and inhibit the inflammatory reactions with good curative effect.