枸杞复方颗粒制剂对紫外线诱导的黄褐斑小鼠皮肤损伤的防治效果研究
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R285.5

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宁夏回族自治区重点研发计划项目(2017BY079);宁夏回族自治区重点研发计划项目(2021BEF02011);宁夏回族自治区重点研发 计划项目(2021BEF02013)

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    摘要:

    目的:观察枸杞复方颗粒制剂防治紫外线(UVB) 诱导的黄褐斑小鼠皮肤损伤的效果。方法: 40 只ICR 雌性小鼠随机分为正常组、模型组、阳性组及中药组,每组10 只。造模前1 d 开始药物干预,阳性 组灌胃100 mg/kg 维生素C 水溶液;中药组灌胃9.3 g/kg 枸杞复方颗粒制剂水溶液;正常组和模型组灌胃等体 积0.9%氯化钠溶液;连续干预15 d。药物干预第2 天除正常组外,其余各组小鼠连续14 d 照射UVB(每天 2 次,每次1 h) 诱导小鼠形成黄褐斑。采用苏木素-伊红(HE) 染色观察小鼠皮肤组织病理变化;免疫组织 化学法观察小鼠皮肤黑色素表达;分光光度法检测小鼠皮肤组织酪氨酸酶、总抗氧化能力(T-AOC)、总超氧 化物歧化酶(T-SOD)、过氧化氢酶(CAT) 和丙二醛(MDA) 表达。结果:与正常组比较,模型组小鼠皮肤 表现出皱纹和暗沉,表皮上皮细胞、炎症细胞及皮脂腺出现增生,皮肤组织中黑色素、酪氨酸酶和MDA 水平 增加(P<0.05);T-AOC、CAT 和T-SOD 有降低趋势,但差异无统计学意义(P>0.05)。与模型组比较,阳 性组和中药组小鼠皮肤损伤有所缓解;皮肤组织中黑色素、酪氨酸酶水平降低(P<0.05);阳性组、中药 组小鼠皮肤组织T-AOC、T-SOD 水平升高(P<0.05,P<0.01),中药组小鼠皮肤组织MDA 水平下降,差异 有统计学意义(P<0.05)。结论:枸杞复方颗粒制剂能够有效缓解UVB 诱导的黄褐斑小鼠皮肤损伤,其机制 与降低皮肤组织酪氨酸酶和MDA 水平有关。

    Abstract:

    Abstract:Objective:To observe the effect of Compound Lycii Fructus Granules on the prevention and treatment of skin injury induced by ultraviolet B (UVB) in mice with melasma. Methods:A total of 40 female ICR mice were randomly divided into the normal group, the model group, the positive group and the Chinese medicine group,with 10 mice in each group. On the day before modeling,the positive group was given 100 mg/kg aqueous solution of vitamin C by gastric irrigation;the Chinese medicine group was given 9.3 g/kg aqueous solution of Compound Lycii Fructus Granules by gastric irrigation;the normal group and the model group were given an equivalent volume of 0.9% sodium chloride solution by gastric irrigation. The intervention lasted for 15 consecutive days. On the second day of drug intervention,except for the normal group,the other groups were irradiated with UVB for 14 consecutive days (twice a day,with one hour for each time) to induce melasma. The pathological changes in skin of mice were observed by HE staining;the expression of melanin in skin of mice was observed by immunohistochemistry; the expressions of tyrosinase,total antioxidant capacity (T- AOC),total superoxide dismutase (T- SOD),catalase (CAT) and malondialdehyde (MDA) in skin tissue of mice were detected by spectrophotometry. Results: Compared with the normal group,the skin of mice in the model group showed wrinkles and dullness;the epidermal epithelial cells,inflammatory cells and sebaceous glands appeared hyperplasia;and the levels of melanin, tyrosinase and MDA in skin tissue were increased (P<0.05);T-AOC,CAT and T-SOD showed a trend of decrease,but the difference showed no significant difference (P>0.05). Compared with that in the model group,skin injury of mice in the positive group and the Chinese medicine group was relieved;the levels of melanin and tyrosinase in skin tissue were decreased (P<0.05);the levels of T- AOC and T- SOD in skin tissue of mice in the positive group and the Chinese medicine group were increased (P<0.05,P<0.01); the MDA level in skin tissue of mice in the Chinese medicine group was decreased,the difference being significant (P<0.05). Conclusion: Compound Lycii Fructus Granules can effectively relieve skin injury induced by UVB in mice with melasma,and its mechanism is related to reducing the levels of tyrosinase and MDA in skin tissue.

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陆敏,魏炜,常晓婕,刘宁,苏为科,余建强.枸杞复方颗粒制剂对紫外线诱导的黄褐斑小鼠皮肤损伤的防治效果研究[J].新中医,2023,55(3):1-6

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  • 在线发布日期: 2023-02-17
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