钩藤碱对惊厥持续状态幼年大鼠血清SOD水平和海马组织TLR4表达的影响
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Rhynchophylline Has Effect on Level of Serum SOD and Expression of TLR4 in Hippocampus of Young Rats after Status Convulsion
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    摘要:

    目的:观察钩藤碱对惊厥持续状态幼年大鼠血清超氧化物歧化酶(SOD) 活性和海马组织Toll样受体4(TLR4)表达的影响。方法160只幼年SD雄性大鼠随机分成正常组、模型组和钩藤碱低、中、高各剂量组,每组32只,各组再按不同时间点分为4 h、24 h、48 h和72 h 4个亚组。采用腹腔注射氯化锂-匹鲁卡品复制幼年大鼠惊厥持续状态模型,钩藤碱低、中、高剂量组于造模后分别给予钩藤碱[10、20、40 mg/(kg·d)]腹腔注射,每天1次,连用3天,正常组、模型组腹腔注射等体积生理盐水。羟胺法检测大鼠血清中SOD活性,免疫组化法检测大鼠海马组织中TLR4的表达情况。结果:模型组大鼠惊厥后4 h时血清SOD活性降低,海马组织TLR4的表达增加,48 h达到峰值,4个时间点模型组大鼠血清SOD值均低于正常组,海马组织TLR4的表达均高于正常组(P<0.01)。与模型组比较,钩藤碱各剂量组大鼠血清SOD活性均显著升高,以24 h、48 h和72 h时较明显;钩藤碱各剂量组大鼠海马组织TLR4的表达均显著降低(P<0.05,P<0.01),钩藤碱各剂量组大鼠血清SOD活性随着钩藤碱剂量增大而增强,海马组织TLR4的表达随着钩藤碱剂量增大而减少。结论:钩藤碱可增加惊厥持续状态幼年大鼠血清SOD活性,下调海马组织TLR4的表达,提示钩藤碱可能对持续惊厥引起的脑损伤有保护作用。

    Abstract:

    Objective: To observe the effect of rhynchophylline on activity of serum superoxide dismutase(SOD)and expression of Toll-like receptors 4(TLR4)in hippocampus of young rats after status convulsion.Methods:Divided 160 male SD young rats randomly into the normal group, the model group, as well as the low, middle, and high dose of rhynchophylline groups,32 rats in each group.Each group was further divided into four subgroups according to different time points: 4 h subgroup, 24 h subgroup, 48 h subgroup and 72 h subgroup.Adopted intraperitoneal injection of lithium chloride-pilocarpine to replicate the model of young rats after status convulsion.After the establishment of model,the low,middle,and high dose of rhynchophylline groups were respectively given 10 mg/(kg·d),20 mg/(kg·d)and 40 mg/(kg·d)of rhynchophylline by intraperitoneal injection once a day for 3 days.The normal group and the model group receivedintraperitoneal injection of the same volume of saline.Activity of serum SOD of rats was detected by hydroxylamine method and expression of TLR4 in hippocampus of rats was detected by immunohistochemical method.ResultsActivity of serum SOD of rats in the model group was decreased at 4 h after convulsion,while expression of TLR4 in hippocampus of rats was increased and reached the peak at 48 h.Activity of serum SOD of rats in the model group at 4 time points was respectively lower than that in the normal group,while expression of TLR4 in hippocampus of rats in the model group was higher than that in the normal group(P<0.01).Comparing with that in the model group,activity of serum SOD of rats in each dose group of rhynchophylline was increased obviously and was most obviously at 24 h,48 h and 72 h.Expression of TLR4 in hippocampus of rats in each dose group of rhynchophylline was obviously decreased(P<0.05,P<0.01).Activity of serum SOD of rats in each dose group of rhynchophylline was enhanced with the increase of concentration of rhynchophylline,while expression of TLR4 in hippocampus of rats was decreased with the increase of concentration of rhynchophylline.Conclusion:Rhynchophylline can increase activity of serum SOD and down-regulate expression of TLR4 in hippocampus of young rats after status convulsion,indicating that rhynchophylline may have protective effect on brain injury caused by status convulsion.

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王常普,蔡小平.钩藤碱对惊厥持续状态幼年大鼠血清SOD水平和海马组织TLR4表达的影响[J].新中医,2018,50(3):6-9

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  • 在线发布日期: 2018-03-04
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