清肝方对脂肪肝大鼠氧化损伤相关因子及LXR-ɑ 表达的影响
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Qinggan Prescription Has Effect on Oxidative Damage Factor and LXR-α Expression of Rats with Fatty Liver
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    摘要:

    目的:探讨清肝方对脂肪肝大鼠的疗效及对氧化损伤相关因子及肝X受体α基因(LXR-α)表达的影响。方法:将60只SD大鼠随机分成正常组、模型组、清肝方低剂量组、清肝方中剂量组、清肝方高剂量组、辛伐他汀组,每组10只。除了正常组,其余各组采用基础饲料、2%胆固醇及10%猪油喂养6周,复制大鼠脂肪肝模型。辛伐他汀组灌胃辛伐他汀25.2 mg/(kg·d);清肝方低、中、高剂量组分别灌胃清肝方5、10、15 g/(kg·d),正常组和模型组同期予等容量生理盐水灌胃;每天1次,连续给药6周。比较各组血脂水平和肝功能。检测各组总抗氧化能力(T-GSH)、超氧化物歧化酶活性(SOD)、丙二醛(MDA)水平和LXR-α mRNA的表达。结果:与正常组比较,模型组大鼠总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、LXR-α mRNA、MDA水平明显升高,高密度脂蛋白胆固醇(HDL-C)、SOD、T-GSH水平明显降低,差异均有统计学意义(P<0.01)。与模型组比较,清肝方低、中、高剂量组和辛伐他汀组大鼠TC、TG、LDL-C、FFA、ALT、AST、LXR-α mRNA、MDA水平明显降低,HDL-C、SOD、T-GSH明显升高,差异均有统计学意义(P<0.05,P<0.01)。结论:清肝方对脂肪肝模型大鼠具有明显改善血脂和肝功能作用,通过提高肝脏的脂质过氧化产物清除能力,降低LXR-α的表达来改善肝血脂和肝功能。

    Abstract:

    Objective:To investigate the clinical effect of Qinggan prescription on rats with fatty liver and its effect on oxidative damage factor and expression of Liver X Receptor(LXR)-α.Methods:Divided 60 SD rats into the normal group,the model group,the group of low-dose Qinggan prescription,the group of medium-dose Qinggan prescription,the group of high-dose Qinggan prescription,and the simvastatin group randomly,10 rats in each group.Except the normal group,all groups were given barley-based diets,2%of cholesterol,and 10%of lard to feed rats for six weeks.Fatty liver models of rat were duplicated.The simvastatin group was given 25.2 mg/(kg·d)of simvastatin by gavage.Qinggan prescription of low,middle and high dose groups were respectively given 5,10,and 15 g/(kg·d)of Qinggan prescription by gavage,while the normal group and the model group were given equivalent normal saline by gavage,once a day,lasting for six weeks.Compared blood lipid level and liver function in each group.Detected total antioxidant capacity(T-GSH),superoxide dismutase activity(SOD),level of malondialdehyde(MDA),and expression of LXR- α mRNA in each group.Results:Compared with the normal group,total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),free fatty acid(FFA),alanine aminotransferase(ALT),aspartate aminotransferase(AST),LXR- α mRNA,and levels of MDA in the model group were all obviously increased, while high-density lipoprotein cholesterol(HDL-C), SOD, and levels of T-GSH were all obviously decreased,differences being significant(P < 0.01).Compared with the medium group,TC,TG,LDL-C,FFA,ALT,AST,LXR-α mRNA,and levels of MDA in the Qinggan prescription of low,medium and high dose groups and the simvastatin group were all evidently decreased,differences being significant(P< 0.05,P< 0.01).Conclusion:Qinggan prescription can significantly improve blood lipids and liver function in fatty liver model of rats,and improve hepatic lipid and liver function by promoting the ability of liver to remove lipid peroxidation products and to reduce the expression of LXR-α.

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高传鹏,刘钰军,邓秀琼.清肝方对脂肪肝大鼠氧化损伤相关因子及LXR-ɑ 表达的影响[J].新中医,2018,50(4):5-9

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  • 在线发布日期: 2018-04-10
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