紫癜灵对慢性免疫性血小板减少症患者Trp代谢的影响
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R558+.2

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广东省中医药局资助项目(20142050);广东省中医院院内专项(YN2014ZHR204)


Effect of Zidianling on Trp Metabolism in Patients with Chronic Immune Thrombocytopenia
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    摘要:

    目的:观察紫癜灵对慢性免疫性血小板减少症(ITP) 阴虚血热证患者吲哚胺2,3-双加氧酶(IDO)/色氨酰-tRNA合成酶(TTS) 介导的色氨酸(Trp) 代谢途径的影响。方法:选取健康志愿者(正常对照组) 与慢性ITP阴虚血热证患者(患者组)各20例。正常对照组不做任何处理,慢性ITP阴虚血热证患者采用紫癜灵治疗。治疗前后分别采集患者的肝素抗凝血标本,检测CD4+及CD8+T淋巴细胞中IDO、TTS表达及血浆Trp、Kyn浓度。结果:完全反应10例,有效7例,无效3例,有效率85.0%。患者组治疗前CD4+、CD8+T淋巴细胞IDO的平均荧光强度低于正常对照组(P<0.05),TTS的平均荧光强度高于正常对照组(P<0.05)。治疗后,患者组CD4+、CD8+T淋巴细胞中IDO的平均荧光强度较治疗前上升(P<0.05),TTS的平均荧光强度较治疗前下降(P<0.05)。患者组治疗前血浆Trp及Kyn浓度均高于正常对照组,差异均有统计学意义(P<0.05)。治疗后,患者组Trp浓度较治疗前降低,差异有统计学意义(P<0.05);Kyn浓度较治疗前升高,差异有统计学意义(P<0.05)。结论:紫癜灵可能通过影响慢性ITP阴虚血热证患者体内IDO/TTS介导的Trp代谢途径发挥其治疗作用。

    Abstract:

    Objective: To observe the effect of Zidianling on the metabolicpathway of tryptophan(Trp)mediated by indoleamine 2,3-dioxygenase(IDO)/tryptophanyl-tRNA synthetase(TTS)in patients with chronic immune thrombocytopenia(ITP)with yin-deficiency and blood-heat syndrome.Methods:Selected healthy volunteers(the normal control group)and patients with chronic ITP with yin-deficiency and blood-heat syndrome(the patient group),20 cases in each group.The normal control group received no treatment,while the patient group received Zidianling for treatment.Before and after treatment,collected heparin anticoagulant specimens of patients,and detected the expression of IDO and TTS in CD4+and CD8+T of T lymphocytes as well as the concentration of Trp and Kyn in plasma.Results:There were 10 cases of complete reaction,in which 7 cases were effective and 3 cases were ineffective,with the total effective rate being 85.0%.Before treatment,the mean fluorescent intensity of IDO in CD4+and CD8+T of T lymphocytes in the patient group was lower than that in the normal control group(P < 0.05),and the mean fluorescent intensity of TTS was higher than that in the normal control group(P < 0.05).After treatment,the mean fluorescent intensity of IDO in CD4+and CD8+T of T lymphocytes in the patient group was higher than that before treatment(P < 0.05),and the mean fluorescent intensity of TTS was lower than that before treatment(P < 0.05).Before treatment,the concentration of Trp and Kyn in plasma in the patient group was higher than that in the normal control group,differences being significant(P < 0.05).After treatment,the concentration of Trp in the patient group was lower than that before treatment,difference being significant(P < 0.05),and the concentration of Kyn in the patient group was higher than that before treatment,difference being significant(P < 0.05).Conclusion:Zidianling may exert its clinical effect by influencing the metabolic pathway of Trp mediated by IDO/TTS in chronic ITP patients with yin-deficiency and blood-heat syndrome.

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高云龙,卓兰云,陈瑶,陈秋杰,马艳,黄宪章,吴新忠.紫癜灵对慢性免疫性血小板减少症患者Trp代谢的影响[J].新中医,2018,50(4):162-165

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  • 在线发布日期: 2018-04-10
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