玉屏风散加味方对慢性阻塞性肺疾病大鼠气道部分细菌定植量的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

R563

基金项目:

国家自然科学基金项目(81460684);云南省科技计划青年项目(2015FD035)


Modified Yup ing feng Powder Prescription Has Effect on Partial Bacterial Colonization in the Airway of Rats with Chronic Obstructive Pulmonary Disease
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:应用玉屏风散加味方干预慢性阻塞性肺疾病(COPD)大鼠,观察其对COPD大鼠气道链球菌、奈瑟菌、葡萄球菌、黄杆菌属、柠檬酸细菌属、弯曲菌属定植量的影响,探讨该方可能的作用机制。方法:选用SPF级雄性Wistar大鼠50只,随机分为正常组、模型组、玉屏风散加味方低、中、高剂量组,每组10只。除正常组外,其余各组用脂多糖(LPS)加烟熏的方法建立 COPD 大鼠模型。玉屏风散加味方低、中、高剂量的浓度分别为 4.644g/(kg·d)、9.288 g/(kg·d)、27.864 g/(kg·d)。采用高通量测序检测分析每组大鼠肺泡灌洗液链球菌、奈瑟菌、葡萄球菌、黄杆菌属、柠檬酸细菌属、弯曲菌属6类细菌的相对含量,苏木素-伊红(HE)染色观察右肺组织病理学变化。结果:与正常组比较,模型组大鼠气道链球菌、奈瑟菌、葡萄球菌、黄杆菌属、柠檬酸细菌属、弯曲菌属相对含量均明显升高,差异有统计学意义( P <0.05)。与模型组比较,玉屏风散加味方低剂量组大鼠气道链球菌、奈瑟菌、弯曲菌属、黄杆菌属、葡萄球菌相对含量明显降低( P <0.05);玉屏风散加味方中剂量组大鼠气道柠檬酸细菌属、黄杆菌属、葡萄球菌相对含量明显降低( P <0.05);而玉屏风散加味方高剂量组大鼠气道6类细菌相对含量的差异无统计学意义(P>0.05)。结论:玉屏风散加味方低、中剂量能降低COPD大鼠气道部分细菌的定植,但高剂量时则无明显降低作用。

    Abstract:

    Objective: To app ly modified Yupingfeng powder prescription to intervene rats with chronic obstructive pulmonary disease(COPD),observe its effect on the bacterial colonization of streptococcus, Neisseria, staphylococcus,flavobacterium,Citrobacter and campylobacter in the airway of COPD rats,and discuss the probable functional mechanism of the prescription.Methods:Selected 50 male Wistar rats in SPF grade,and divided theminto the normal group,the model group, the low-dose modified Yupingfeng powder prescription group, the middle-dose modified Yupingfeng powder prescription group and the high-dose modified Yupingfeng powder prescription group,10 rats in each group.Except the normal group,the other groups were given the method of lipopolysaccharide and smoking to establish the model of COPD rats.The concentration of modified Yu ping feng powder prescription in the low-dose,midd le-dose and high-dose group was 4.644 g/(kg·d),9.288 g/(kg·d)and 27.864 g/(kg·d)respectively.By adopting high-throughput sequencing,detected and analyzed the relative content of 6 types of bacteria including streptococcus,Neisseria,staphylococcus,flavo bacterium,Citrobacter and campylobacter in bronchoalveolar lavage fluid of rats in each group.By means of hematoxylin-eosin(HE)staining,observed the histopathologic changes of right lung tissue.Results:Comparing with the normal group,the relative content of streptococcus,Neisseria,staphylococcus,flavobacterium,Citrobacter and campylobacter in the airway of rats in the model group was all significantly increased,difference being significant(P < 0.05).Comparing with the model group,the relative content of streptococcus,Neisseria,campylobacter,flavobacteriumand staphylococcus in the airway of rats in the low-dose modified Yupingfeng powder prescription group was all significantly decreased(P < 0.05);the relative content of staphylococcus,flavobacteriumand Citrobacter in the airway of rats in the middle-dose modified Yupingfeng powder prescription group was all significantly decreased(P < 0.05);but there was no significant difference being found in the relative content of 6 types of bacteria in the airway of rats in the high-dose modified Yupingfeng powder prescription group(P>0.05).Conclusion:Modified Yupingfeng powder prescription in lowand middle dose can decrease the partialbacterial colonization in the airway of COPD rats,but the high-dose one has no significant clinicaleffect in reduction.

    参考文献
    相似文献
    引证文献
引用本文

彭静,羊忠山,李玉卿,袁嘉丽.玉屏风散加味方对慢性阻塞性肺疾病大鼠气道部分细菌定植量的影响[J].新中医,2018,50(7):1-4

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2018-07-09
  • 出版日期: