Objective：To discuss the effect ofmedicated serumof Dangui Buxue tang on apoptosis protein and oxidative damage of ovarian granulosa cells in rats with Cisplatin injury.Methods：Different dosage of medicated serumof Dangui Buxue tang was applied to ovarian granulosa cells in rats in vitro inhibited by 5 μg/mL Cisplatin(CDDP).MTS(3-(4，5-dimethylthiazol-2-yl)-5-(3-carboxy methox yphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)method was used to detect the proliferation of granulosa cells in each group at the 24 th ，48 th and 72 nd hour， Malondialdehyde(MDA)and the expression of Caspase-3 protein in culture medium.Results：CDDP had obvious inhibitory effect on ovarian granulosa cells in rats，and the apoptosis rate can reach 70.50%.Medicated serumof Dangui Buxue tang can promote the proliferation of granulosa cells，and the effect of 2.5%medicated serum of DanguiBuxue tang on the 48 th hour group was the most obvious(P ＜ 0.01)；MDA and Caspase-3 protein decreased gradually at the same time，the difference being significant( P ＜0.01).Compared with that in the Dangui Buxue tang groups of low-dose and high-dose，the curative effect in the Dangui Buxue tang group of middle-dose was the most obvious，the difference being significant( P ＜0.01).Conclusion：Dangui Buxue tang can promote the proliferation of ovarian granulosa cells in rats with Cisp latin injury.Its mechanis mprobably is to inhabit the production of oxygen free radicals of cells and the expression of Caspase-3 protein，and promote the proliferation of cells.