Objective：To optimize the preparation technology of Binghe lotion by anti-inflammatory and sensitization tests.Methods：Selected the same medicinal materials but adopted different extraction technologies to prepare Binghe lotion.Used the method of ear edema in mice induced by xylene，and skin sensitization test in guinea pigs induced by the external application of 2，4-dinitrochloro benzene to screen the optimal preparation technology of Binghe lotion.Results：Comparing with the blank controlgroup，the degree of ear edema ofmice in the group of Binghe lotion by water extraction，the group of Binghe lotion by alcoholextraction，the group of Binghe lotion partly by water extraction and alcoholextraction and the group of Binghe lotion by volatile oil extraction was significantly dcreased respectively，and the inhibition rate of ear edema was significantly increased，differences being significant( P ＜ 0.05， P ＜ 0.01).The sensitization rate was 100%in the modelgroup，while the sensitization rate was 0 in the group of Binghe lotion by water extraction，the group of Binghe lotion by alcohol extraction，the group of Binghe lotion partly by water extraction and alcohol extraction and the group of Binghe lotion by volatile oil extraction.Comparing with the blank control group，the sensitization rate of guinea pigs in the model group was relatively higher，difference being significant(P ＜ 0.01).Comparing with the model group，the sensitization rate of guinea pigs was relatively lower in the group of Binghe lotion by water extraction，the group of Binghe lotion by alcohol extraction，the group of Binghe lotion partly by water extraction and alcoholextraction and the group of Binghe lotion by volatile oilextraction，differences being significant( P ＜0.01).Conclusion：Volatile oilextraction is the optimalpre paration technology of Binghe lotion.