Objective：To observe the effect of liver disease on gallbladder through ultrasonography，so as to discuss the authenticity of the theory of the interrelation between liver and gallbladder in Chinese medicine and provide clinical reference for the basic theory of Chinese medicine.Methods：Through case-control studies，analyzed the ultrasonographic changes of gallbladder in patients with liver disease or without liver disease so as to summarize the effect of liver disease on the ultrasonographic images of gallbladder.Results：There were 147 cases of patients aged 22 to 92 in the experiment group，including 6 cases of primary liver cancer，8 cases of liver cirrhosis，51 cases of fatty liver，37 cases of liver cyst，18 cases of enlargement of the echo in liver，13 cases of liver hemangioma，and 14 cases of in trahepatic bile duct stone.There were 300 cases of patients aged 23 to 92 in the normal control group，with an average age of 54.The incidence of abnormal ultrasonic image of gallbladder was 38.1%in the experiment group and 17.0%in the normal control group，the difference being significant(P ＜ 0.01).The incidence of gallbladder stones，gallbladder polyps，rough gallbladder wall and thickened edema of gallbladder was higher than that in the controlgroup( P ＜0.05).In the experiment group，the incidence of abnormal ultrasonic image of gallbladder was the highest in patients with primary liver cancer(100%).The incidence of abnormal ultrasonic image of gallbladder ranked as follows：primary liver cancer＞liver cirrhosis＞enlargement of the echo in liver＞fatty liver＞intrahepatic bile duct stone＞liver cyst＞liver hemangioma.The incidence of abnormal ultrasonic image of gallbladder in patients with primary liver cancer，liver cirrhosis，enlargement of the echo in liver and fatty liver was obviously higher than that in normal patients( P ＜0.01).The incidence of abnormal ultrasonic image of gallbladder in patients with liver cyst，liver hemangioma and intrahepatic bile duct stone was similar to that in normal patients(P＞0.05).Conclusion：Liver disease and gallbladder disease are closely related to each other，and the occurrence of primary liver cancer，liver cirrhosis，enlargement of the echo in liver and fatty liver has a certain correlation with the occurrence of gallbladder disease.However，the theory of the interrelation between liver and gallbladder in Chinese medicine does not apply to all liver disease.