Objective： To observe the clinical effect of 1540nmnon-ablative fractional laser combined with Xiaoban tincture for alopecia areata.Methods：Divided 90 cases of patients with alopecia areata into three groups according to randomized controlled method.The Xiaoban tincture group was treated with simple Xiaoban tincture，while the laser group was treated with simple 1540 nmnon-ablative fractional laser，and the combination group was treated with the combined treatment of the above two groups.The treatment of the three groups lasted for eight weeks.Observed the changes of alopecia area and clinical effect in the three groups before and after treatment.Results：The total effective rate of clinical effect was 83.3% in the combination group， 60.0% in the Xiaoban tincture group and 53.3% in the laser group， the difference being significant( P ＜0.05).The clinical effect in the combination group was superior to that in the Xiaoban tincture group and the laser group.After treatment， alopecia area of the three groups decreased；when compared alopecia area between the three groups，differences were significant( P ＜0.05).The differences between any two groups were statistically significant( P ＜0.05).The decrease of alopecia area of the combination group was better than that of the Xiaoban tincture group and the laser group.No adverse reaction was found in the three groups.Conclusion：The therapy of 1540nmnon-ablative fractional laser combined with Xiaoban tincture for alopecia areata is superior to that of simple treatment of laser or Xiaoban tincture.