Objective：To study the intervention effects of Zhenw u tang and its decomposed recipes on the oxidative dam age of renal tubular epithelial cells and to provide experimental evidence for the scientific character of principles of the Chinese medicine treatment.Methods：Used different concentrations of H 2 O 2 to act on renal tubular epithelial cells HK-2 toform a model of the oxidative dam age in renal tubular epithelial cells.Intervened by the group of Zhenw u tang in serum containing drug,the group forwarming yang in serum containing drug and the group for promoting diuresis in serum containing drug,and detected the proliferation effect in injured cells in each Chinese medicine group as well as the effects on the expressions of hypoxia-injured genes(HO-1，HIF-1α and survivin)respectively.Detected the effects on renal fibrosis and the expressions of relative genes of renal tubular epithelial transdifferentiation，such as TGF-β1，SMA and E-cadherin.Results：H 2 O 2 can cause significant dam ages to HK-2 cells at different times.Compared with the model group，the group of Zhenw u tang and the group for promoting diuresis had a significant effect in repairing HK-2 cells( P ＜0.05)，while the group for warming yang had no obvious effect.The group of Zhenw u tang，the group for warming yang and the group for promoting diuresis can elevated the expressions of HO-1，HIF-1α genes and had a significant effect in inhibiting survivin genes.when compared with the model group( P ＜0.05)，but the differences among the three groups were not evident.The group of Zhenw u tang，the group of warming yang and the group for promoting diuresis can reduce the expressions of SMA and E-cadherin genes；and the group of warming yang can reduce the expressions of TGF-β1，differences being significant( P ＜0.05).Conclusion：Zhenwu tang has an obvious effect in protecting the model of oxidative dam age renal tubular epithelial cells in vitro.the group for promoting diuresis has a more significant effect in repairing the injured modeland the group for warming yang has an evident effect in ameliorating renal fibrosis and renal tubular epithelial transdifferentiation.This study provides an experimental basis for the scientific character of principles of Chinese medicine treatment.