Objective： To observe the clinical effect of spleen-enforcing and kidney-nourishing method com bined with glisamuride for patients with latent autoimmune diabetes in adults(LADA).Methods：Divided 90 cases of LADA patients with spleen and kidney deficiency syndrome into the control group，the control group of glisamuride and the treatment group of integrated Chinese and western medicine randomly，30 cases in each group.The controlgroup was given insulin，the control group of glisamuride received insulin com bined with glisamuride tablets，and the treatment group of integrated Chinese and western medicine was given insulin，glisamuride tablets as wellas Pishen prescription.The intervention in the three groups all lasted for 36 weeks.Detected the levels of glycosylated hemoglobin，fasting C-peptide，glucagon-like peptide-1(GLP-1)and glucagon before treatment and after 12， 24， and 36 weeks of treatment respectively.Results： After 24 weeks of treatment，the levels of glycosylated hemoglobin in the three groups were all decreased when compared with those before treatment，differences being significant( P ＜0.05).After 36 weeks of treatment，glycosylated hemoglobin level in the treatment group of integrated Chinese and western medicine was lower than those in the control group and the controlgroup of glisamuride，differences being significant( P ＜0.05).After 12 weeks of treatment，fasting C-peptide levels in the threegroups were all increased when compared with those before treatment，differences being significant( P ＜0.05).The C-peptide levels in the controlgroup of glisamuride and the treatment group of integrated Chinese and western medicine after 24 weeks of treatment were increased when compared with those in the same groups after 12 weeks of treatment，differences being significant( P ＜0.05).After 36 weeks of treatment，C-peptide levels in the controlgroup of glisamuride and the treatment group of integrated Chinese and western medicine were increased when compared with those after 24 weeks of treatment，differences being significant( P ＜0.05)and were higher than that in the control group during the same period，differences being significant( P ＜0.05).C-peptide level in the treatment group of integrated Chinese and western medicine was higher than that in the controlgroup of glisamuride，difference being significant( P ＜0.05).After 24 weeks of treatment，GLP-1 levels in the control group of glisamuride and the treatment group of integrated Chinese and western medicine were increased when compared with that in the controlgroup during the same period，differences being significant( P ＜0.05).After 36 weeks of treatment，GLP-1 level in the treatment group of integrated Chinese and western medicine was higher than those in the control group of glisamuride and the control group，differences being significant( P ＜0.05).Glucagon in the control group of glisamuride and the treatment group of integrated Chinese and western medicine were decreased when compared with those before treatment，differences being significant( P ＜0.05).Conclusion：The therapy of the formula of spleen-enforcing and kidney-nourishing method com bined with glisamuride can significantly increase the levels of GLP-1 and C-peptide in LADA patients and inhibitα cells from reducing glucagon secretion.Expected to alleviate the natural history of LADA，it is beneficial to the recovery ofβ cells function and is worthy of further study.