Objective：To observe the effect of Tang Shen Kang prescription(TSK)on the blood glucose，blood lipid，24h urinary protein and morphology of kidney tissues in db/db mice.Methods：Twelve-week-old male db/db mice at SPF level were randomly divided into the blank group，the model group(the db/db group)，the TSK groups of high，middle and low dose.They were fed for 12 weeks.Recorded the levels of body mass，blood lipid，blood glucose and 24h urinary protein，and observed the pathological changes of kidney.Results：Compared with the blank group，in the model group，the levels of body mass and the blood glucose were increased，and the level of 24h urinary protein maintained high(P＜0.05)；hyperplasia of glomerular mesangial matrix occurred.Compared with the model group，the body weight of db/db mice in the TSK groups were decreased；as the prolongation of treatment time，the levels of blood glucose，blood lipid and 24h urinary protein were significantly decreased in the TSK groups of high and middle dose(P＜0.05)；pathological changes of kidney tissues were improved.Conclusion：TSK can protect the kidney of db/db mice.Its mechanism is related to the reduction of blood lipid and blood glucose and the improvement of glucotoxicity and lipid toxicity，which can improve the proliferation of glomerular mesangium.