Objective：To compare the content of total flavonoids in wild phellinus igniarius from different producing areas.Methods：Used 70%ethanol to reflux and extract total flavonoids from wild phellinus igniarius from different places，and purified total flavonoidsby using AB-8 macroporousresin，and determined thecontent of total flavonoidsby UV spectrophotometry.Rutin was used as the reference substance with a wave length of 507 nm.Results：The content of total flavonoids in wild phellinus igniarius from Changbai Mountain of Jilin Province，Xiajin of Shandong Province，Changdu of Tibet， Lincang of Yunnan Province， Jinzhai of Anhui Province and Ganzi of Sichuan Province was62.22， 55.84，60.08，37.64，48.63 and 39.90 mg/g，respectively.Conclusion：The established method can determine the total flavonoids in samples accurately and quickly.The content of total flavonoids in wild phellinus igniarius was different in different producing areas.