Objective：To explore the effect of Buzhong Yiqi tang on the expression of protein and mRNA of Fibulin-5 in grade-III prolapsed hemorrhoid tissuesof patients.Methods：Divided 59 casesof patientswith grade-III prolapsed hemorrhoid who were differentiated with syndrome of sinking of qi due to spleen deficiency in outpatient number order into three groups according to random number table，20 cases in the treatment group(Group A)，20 cases in the control group(Group B)，and 19 cases in the blank group(Group C).Group A was treated with melilotus extract tablets orally and Buzhong Yiqi tang；Group B was simply treated with oral administration of melilotus extract tablets；Group C was given no medication.All groups were treated for one week.On the second day after treatment，respectively selected 10 cases of hemorrhoid tissue samples of patients and divided them into three groups， namely Group A1， B1 and C1.Adopted PCR and Western-blot technology to systematically study and compare the difference of the expression of protein and mRNA of Fibulin-5 in the hemorrhoid tissues of patients in each group from the gene-protein levels.Results：The Fibulin-5 dissolution curve and the β-Actin dissolution curve indicated that the primer was specific and had no stray band or noise signal.Compared the expression level of mRNA of Fibulin-5 between the three groups，the difference was significant(P＜0.05).In pairwise comparison between groups，the expression level of mRNA of Fibulin-5 in Group A1 was higher than that in Group B1(P＜0.05)； the one in Group A1 was higher than that in Group C1(P＜0.05)；the one in Group B1 was higher than that in Group C1(P＜0.05).From the protein band of Fibulin-5，it was found that the expression of protein of Fibulin-5 was the strongest and concentrated in Group A1(P＜0.05).Compared the expression level of protein of Fibulin-5 between the three groups，the difference was significant(F=12.581，P= 0.000 ＜ 0.05).In pairwise comparison between groups，the expression level of protein of Fibulin-5 in Group A1 was higher than that in Group B1(P＜0.05)；the one in Group A1 was higher than that in Group C1(P＜0.05)；the one in Group B1 was similar with that in Group C1，there being no significance in the difference(P＞0.05).Conclusion：Buzhong Yiqi tang can improve the expression of protein and mRNA of Fibulin-5 in grade-III prolapsed hemorrhoid tissues.The decrease of expression of protein and mRNA of Fibulin-5 may be one of the pathological mechanisms that causes prolapsed hemorrhoid.