Objective：To observe the clinical effect of Bushen Huoxue tang combined with sodium hyaluronate for knee osteoarthritis(KOA)，and its effect on the levels of C-telopeptide of type I collagen(CTX-I)and C-telopeptide of type II collagen(CTX-II)in serum.Methods：Divided 120 cases of KOA patients with kidney deficiency and blood stasis syndrome into the control group and the observation group randomly，60 cases in each group.Both groups received sodium hyaluronate via injection and underwent functional exercise in the same period，while the observation group additionally received Bushen Huoxue tang via oral administration.The treatment of both groups lasted for 4 weeks.Before and after treatment，evaluated knee joint function via the Western Ontario and McMaster Universities(WOMAC)Osteoarthritis Index，assessed the degree of pain via Visual Analogue Scale(VAS)，and detected the levels of CTX-I and CTX-II in serum in both groups.Results：After treatment，the clinical effect in the observation group was better than that in the control group，difference being significant(P＜0.05).The scores of pain，stiffness and joint function and the total score in WOMAC and the scores of VAS of patients in both groups were respectively lower than those before treatment(P ＜ 0.01)；each score above in the observation group was lower than that in the control group(P＜0.01).The levels of CTX-I and CTX-II in serum in both groups were lower than those before treatment(P ＜ 0.01)，the levels of CTX-I and CTX-II in serum in the observation group were lower than those in the control group.Conclusion：The application of Bushen Huoxue tang combined with injection of sodium hyaluronate in articular cavity can improve the knee joint function of patients with knee osteoarthritis，alleviate pain and promote clinical effect.One of its functional approaches may be regulating such bone metabolic markers as CTX-I and CTX-II.