Abstract：Objective：To observe the effect of puerarin injection on heart function and levels of endothelin- 1(ET-1) and brain natriuretic peptide(BNP) in plasma in patients with acute myocardial infarction. Methods：A total of 94 cases of patients with acute myocardial infarction were selected as the study objects， and were divided into the observation group and the control group according to the random number table method， 47 cases in each group. The control group was treated with routine thrombolysis and percutaneous coronary intervention(PCI)， followed by aspirin enteric- coated tablets， and the observation group was additionally treated with puerarin injection based on the treatment of the control group. Both groups were treated for seven weeks. The heart function indexes and levels of ET-1 and BNP in plasma before and after treatment as well as the clinical effect in the two groups were compared. Results：The total effective rate was 91.49% in the observation group and 82.98% in the control group； there was no significant difference between the two groups(χ 2=1.529， P＞0.05). After treatment， the left ventricular ejection fraction(LVEF) and fraction shortening(FS) in the two groups were increased when compared with those before treatment(P＜0.05)，and left ventricular end- diastolic diameter(LVDd) were reduced(P＜ 0.05)；LVEF and FS in the observation group were higher than those in the control group(P＜0.05)，and LVDd was smaller(P＜ 0.05). After treatment， the contents of ET- 1 and BNP in plasma in the two groups were decreased when compared with those before treatment(P＜0.05)，and the contents in the observation group were lower than those in the control group(P＜ 0.05). Conclusion：Puerarin injection in adjuvant treatment for patients with acute myocardial infarction can improve the heart function and reduce the levels of ET-1 and BNP in plasma.