Abstract： Objective： To observe the effect of integrated Chinese and western medicine nursing on inflammatory factors，gastrointestinal function and negative emotion in patients undergoing surgery of acute appendicitis. Methods：A total of 110 cases of patients with acute appendicitis and undergoing laparoscopic surgery were divided into the control group and the observation group according to the random number table method，55 cases in each group. Both groups were conducted routine laparoscopic appendectomy；the control group was given routine high- quality nursing；the observation group was given integrated Chinese and western medicine nursing. The clinical operation indexes， negative emotion， inflammatory factors， gastrointestinal function， psychological state and postoperative complications were compared between the two groups. Results： The operation time， the bed rest time， the hospitalization time and hospitalization expenses in the observation group were less than those in the control group，differences being significant(P＜0.05). After nursing，the scores of Self- rating Anxiety Scale and Depression Scale in the two groups were decreased when compared with those before treatment， and the two scores in the observation group were lower than those in the control group， differences being significant(P＜0.05). After nursing，the levels of interleukin-6(IL-6)，tumor necrosis factor-α(TNF-α) and C-reactive protein (CRP) in the two groups were decreased when compared with those before treatment，and the three levels in the observation group were lower than those in the control group，differences being significant(P＜0.05). The recovery time of bowel sound， the first exhaust time and the first defecation time in the observation group were lower than those in the control group， differences being significant(P＜0.05). After nursing，the scores of sensation，emotion，behavior，daily living habits，diet habits and sleeping status in the two groups were lower than those before nursing(P＜0.05)， and the six scores in the observation group were significantly lower than those in the control group(P＜0.05). The incidence of complications in the observation group was 9.09% ， lower than that of 23.64% in the control group， the difference being significant(P＜0.05). Conclusion： The model of integrated Chinese and western medicine nursing can improve the clinical operation indexes， negative emotion， and psychological state of patients undergoing surgery of acute appendicitis， alleviate inflammatory response，strengthen the recovery of gastrointestinal function，and reduce complications.