Abstract：Objective：To discuss the effect of Xuebijing injection(XBJI) on the level of interleukin-1β(IL-1β) in myocardial inflammatory response in rats after hypoxia-reoxygenation. Methods：A total of 48 male SD rats were selected and randomly divided into the control group，the balanced perfusion group，the model group，and the XBJI groups of low-dose，mediumdose and high-dose，eight rats in each group. The isolated rat hearts were treated with constant temperature and pressure perfusion via aorta；after perfusion for twice，the rat models with myocardial hypoxia and reoxygenation were established. In the XBJI groups of low-dose，medium-dose and high-dose，during the establishment of rat models，20，40 and 80 mL/L XBJI was added respectively. The concentrations of protein and mRNA of IL- 1β and creatine kinase isoenzyme(CK- MB) in myocardium were detected. Results： There was no significant difference being found in the comparison of the protein concentrations CK-MB and IL-1β in myocardium between the control group and the balanced perfusion group(P＞0.05)，and the relative content of mRNA of IL-1β was increased(P＜0.05). Compared with those in the balanced perfusion group，the protein concentrations of CK-MB and IL- 1β and the relative content of mRNA of IL- 1β in myocardium in the model group were increased(P＜0.05). Compared with those in the model group，the protein concentrations of CK-MB and IL- 1β in the XBJI groups of different dosages were all decreased(P＜0.05)，and the relative content of mRNA of IL-1β in myocardium was decreased in the group of medium- dose(P＜0.05). Compared with those in the XBJI group of medium- dose， the protein concentrations of CK-MB in the groups of low-dose and high-dose were higher(P＜0.05)，and the protein concentration of IL-1β in the XBJI group of high-dose was higher(P＜0.05). Conclusion：XBJI can inhibit the activity of CK-MB，reduce the level of IL- 1β and alleviate myocardial inflammatory response in rats after hypoxia and reoxygenation. The medium dose of XBJI has the best effect.