Abstract：Objective：To observe the application effect of Chinese medicine nursing based on syndrome differentiation in rehabilitation of shoulder pain after stroke. Methods： A total of 120 cases of patients with shoulder pain after stroke were divided into the control group and the observation group according to the random number table method， 60 cases in each group. The control group was given routine nursing，and the observation group was given routine nursing based on syndrome differentiation. The differences of rehabilitation effect between the two groups were analyzed. Results： After intervention， the total effective rate was 91.67% in the observation group，higher than that of 78.33% in the control group，the difference being significant(P＜0.05). Before intervention， there was no significant difference being found in the comparison of the scores of Fugl- Meyer Assessment(FMA) and Barthel Index(BI) between the two groups(P＞0.05). After intervention， the above two scores in the two groups were both higher than those before intervention， the difference being significant(P＜ 0.05)；the two scores in the observation group were higher than those in the control group，the difference being significant (P＜0.05). Before intervention， there was no significant difference being found in the comparison of the scores of Neurological Function Defect Scale(NFD) and Visual Analogue Scale(VAS) between the two groups(P＞0.05). After intervention，the above two scores in the two groups were both lower than those before intervention，the difference being significant(P＜0.05)； the two scores in the observation group were lower than those in the control group， the difference being significant(P＜0.05). Conclusion： The application of Chinese medicine nursing based on syndrome differentiation in rehabilitation of shoulder pain after stroke can achieve good application effect.