Abstract：Objective：To discuss the effect of curcumin on proliferation，migration and invasion of HCCLM3 cell line in vitro and its potential molecular mechanism. Methods：In the blank control group，HCCLM3 cells were treated with 0 μmol/L of curcumin；in the curcumin group 1，HCCLM3 cells were treated with 30 μmol/L of curcumin；in the curcumin group 2，HCCLM3 cells were treated with 60 μmol/L of curcumin. In the three groups， cell proliferation was detected by MTT method， and cell migration and invasion were detected by Transwell method. In the blank control group and the curcumin group 2， the changes of expression of TUFT1 and phosphorylation of AKT were detected by qRT- PCR and Western blot. Results：The experimental results of MTT showed that，compared with that in the blank control group，the proliferation of HCCLM3 cells in the curcumin group 1 and 2 was significantly decreased(P＜0.05). The results of Transwell Migration Assay showed that，compared with that in the blank control group，the migration of HCCLM3 cells in the curcumin group 1 and 2 was significantly decreased(P＜0.05). The results of Transwell Invasion Assay showed that，compared with that in the blank control group，the invasion of HCCLM3 cells in the curcumin group 1 and 2 was significantly decreased(P＜0.05). The results of qRT-PCR showed that，compared with that in the blank control group，the expression level of TUFT1 mRNA in HCCLM3 cells in the curcumin group 2 was significantly decreased(P＜0.05). The results of Western blot showed that，in the curcumin group 2，the expression level of TUFT1 protein and the phosphorylation of AKT in HCCLM3 cells were significantly decreased (P＜0.05). Conclusion： Curcumin may inhibit the proliferation， migration and invasion of hepatocellular carcinoma by inhibiting the activation of TUFT1/AKT signaling pathway.