基于网络药理学探讨四逆散“一方多效”的分子机制
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R285

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广东省自然科学基金自由申请项目(2018A0303130072);国家级大学生创新创业计划项目(201810572009);广州中医药大学科研团队培育项目(2019KYTD301)


Discussion of Molecular Mechanism of “One Prescription with Multiple Effects” in Sini Powder Based on Network Pharmacology
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    摘要:

    目的:运用网络药理学的方法探讨四逆散“一方多效”的分子机制。方法:通过中药系统药理学分析平台(TCMSP) 数据库进行四逆散主要活性成分、相关作用靶点的预测和筛选。借助检索相互作用的基因/蛋白质搜索工具平台(STRING) 构建靶蛋白互作网络,利用BiNGO 和MCODE 插件对靶基因进行基因本体(GO) 生物过程富集和聚类分析,运用注释、可视化和集成发现的数据库(DAVID) 平台对关键靶点京都基因与基因组百科全书(KEGG) 信号通路进行富集分析。结果:从四逆散中共筛选出121种活性成分,并作用于248个潜在靶点,参与炎症免疫调控、体内物质代谢、信号转导等54个主要GO生物过程,调控癌症、免疫炎症、细胞信号转导等137条信号通路。结论:四逆散有“一方多效”的临床应用价值,其不仅对多种免疫炎症疾病(如溃疡性结肠炎、类风湿性关节炎等) 具有很好的疗效,同时也可以有效治疗神经系统疾病、癌症等。

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    Abstract:Objective:To discuss the molecular mechanism of “one prescription with multiple effects” in Sini powder by applying the method of network pharmacology. Methods: The prediction and screening of main active components and related targets of Sini powder were performed by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). The target protein interaction network was constructed by Search Tool for the Retrieval of Interacting Genes/Proteins(STRING); the enrichment and cluster analysis of biological process in Gene Ontology(GO) of target genes were performed by BiNGO and MCODE; the enrichment analysis of key targets in Kyoto Encyclopedia of Genes and Genomes (KEGG)- based pathway was carried on by Database for Annotation, Visualization, and Integrated Discovery(DAVID). Results:A total of 121 kinds of active components were screened from Sini powder,which acted on 248 potential targets,participated in 54 main GO biological processes such as immune regulation of inflammation,material metabolism in vivo and signal transduction,as well as in 137 signal pathways such as cancer regulation,inflammation immunization and cell signal transduction. Conclusion:Sini powder has the clinical application value of "one prescription with multiple effects". It not only has good curative effect in the treatment of inflammatory- immune diseases, such as ulcerative colitis and rheumatoid arthritis,but also can effectively treat nervous system diseases,cancer,etc.

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侯雪楠,张苗,崔赛男,杨蕾,李强.基于网络药理学探讨四逆散“一方多效”的分子机制[J].新中医,2020,52(11):26-31

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  • 在线发布日期: 2020-06-04
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