Abstract：Objective：Based on the distribution rules of qi and blood in meridian and collateral as well as Zang-Fu in the theory of Ziwu Liuzhu， to analyze the blood glucose in patients with type 2 diabetes mellitus in different hours， and to discuss the relationship between the control of blood glucose and Zang-Fu. Methods：Retrospective analysis was conducted in 386 cases of patients who had type 2 diabetes mellitus and were under continuous glucose monitoring. The data of blood glucose were used for statistical calculation of mean blood glucose(MBG)， standard deviation of blood glucose(SDBG) for blood glucose fluctuation， low blood glucose index(LBGI) and high blood glucose index(HBGI) in different hours. The relevance of blood glucose in different hours and that in general was analyzed. Results：The MBG peaked in Si Shi (9 am— 11 am)， the period of the spleen， followed by You Shi (5 pm—7 pm)， the period of the kidney. The highest relevance of MBG in different hours and the general blood glucose throughout a day was appeared in Hai Shi (9 pm—11 pm)，the period of the triple energizers，followed by Yin Shi (3 am—5 am)，the period of the lung. The highest SDBG was in You Shi，the period of the kidney，followed by Shen Shi (3 pm—5 pm)，the period of the bladder. The strongest relevance of SDBG was in Hai Shi，the period of the triple energizers，and Wei Shi (1 pm—3 pm)，the period of the small intestine，followed by Yin Shi， the period of the lung. In Hai Shi，the period of the triple energizers，LBGI was the highest；the strongest relevance of LBGI in different hours and that in general was in Wei Shi，the period of the small intestine. The HBGI reached the highest in Si Shi，the period of the spleen，and the strongest relevance of HBGI in different hours and that in general was in Hai Shi，the period of the triple energizers. Conclusion：The highest MBG and HBGI are in Si Shi，the period of the spleen. The largest blood glucose fluctuation is in You Shi，the period of the kidney. The period of the lung，Yin Shi，is important to MBG，blood glucose fluctuation and HBGI. The period of the triple energizers，Hai Shi is the period of the strongest relevance between MBG，blood glucose fluctuation as well as HBGI in different hours and those throughout a day respectively，and the period of the highest LBGI.