Abstract：Objective：To observe the effect of acupuncture analgesia combined with propofol on pain degree and stress response in patients undergoing enteroscopy. Methods： A total of 160 cases of patients undergoing enteroscopy were selected and divided into the acupuncture group and the control group according to the random number table method， 80 cases in each group. The control group was given propofol for enteroscopy，and the acupuncture group was additionally given acupuncture analgesia based on the treatment of the control group. The pain degree， the dosage of propofol， stress response and satisfaction rates in the two groups were recorded and analyzed. Results： The pain rate was 43.75% in the acupuncture group，lower than that of 80.00% in the control group；the dosage of propofol in the acupuncture group was lower than that in the control group， differences being significant(P＜0.05). Before examination， there was no significant difference being found in the comparison of diastolic blood pressure，systolic blood pressure and heart rates between the two groups(P＞0.05). After examination，diastolic blood pressure，systolic blood pressure and heart rates in the two groups were increased when compared with those before examination； the above indexes in the acupuncture group were lower than those in the control group， differences being significant(P＜0.05). The satisfaction rate was 96.25% in the acupuncture group， higher than that of 73.75% in the control group， the difference being significant(P＜0.05). Conclusion： The application of acupuncture analgesia combined with propofol for enteroscopy can relieve the pain of patients and reduce their stress responses.