Abstract： Objective： To observe the effect of thumbtack needle at ear points combined with Chinese medicine emotional intervention on anxiety，depression and sleep quality of patients with insomnia. Methods：A total of 120 cases of patients with insomnia were randomly divided into two groups， 60 cases in each group. The observation group was given thumbtack needle at ear points combined with Chinese medicine emotional intervention，and the control group was treated with oral administration of estazolam. Both groups were treated for two courses，four weeks being one course. Before and after treatment ， the scores of Pittsburgh Sleep Quality Index(PSQI) ， Self-rating Anxiety Scale(SAS) and Self-rating Depression Scale，as well as the levels of 5-hydroxytryptamine(5-HT) and brain-derived neurotrophic factor(BDNF) in serum were detected. The clinical effect was compared between the two groups. Results：The total effective rate was 93.33% in the observation group and 80.00% in the control group， the difference being significant(P＜0.05). After treatment， the quality，duration，efficiency and disorder of sleep，the time for falling asleep，daytime dysfunction and the total scores in SQI，SAS and SDS in the two groups were all significantly decreased when compared with those before treatment(P＜0.05)， and the above scores in the observation group were lower than those in the control group(P＜0.05). After treatment， the levels of 5-HT and BDNF in the two groups were significantly increased when compared with those before treatment，and the above two levels in the observation group were higher than those in the control group(P＜0.05). Conclusion： The thumbtack needle at ear points combined with Chinese medicine intervention can effectively improve the sleep quality， anxiety and depression of patients with insomnia，whose curative effect is better than that of estazolam.