Abstract:Objective:To discuss the effect of Shenqi Fuzheng injection on apoptosis of the lung cancer cells and Negative Control Region 1/Natural Killer-factor protein46(NCR1/NKp46) signaling pathway. Methods:Lewis cells of human lung cancer were divided into the LEWIS group,the cisplatin group,the Shenqi Fuzheng injection groups of low-dose and high-dose. There were six parallel samples in each hole in each group. In the LEWIS group,10 mL cell sap of lung cancer LEWIS(cell concentration was 5×106/mL) was placed in DMEM culture medium with 10% fetal bovine serum. The cell culture method in the cisplatin group was the same in the LEWIS group,and cisplatin was added to make the final concentration of 50.0 μg/mL. The cell culture methods in the Shenqi Fuzheng injection groups of low- dose and high- dose were the same in the LEWIS group;the two groups were added Shenqi Fuzheng injection accordingly,and the final concentration was 50.0 μg/mL and 100.0 μg/mL respectively;both groups were cultured for 72 hours. After cultivation,MTT method was applied for detecting cell viability; crystal violet staining was carried out to detect the number of monoclonal formation; annexin v- fluorescein isothiocyanate/apoptosis detection kit of propidium iodide was used for detect the level of apoptosis;PT- PCR method and Western blot method were applied for detecting expression levels of gene and protein of NCR1 and NKp46. Results: Compared with those in the LEWIS group,in the cisplatin group and the Shenqi Fuzheng injection groups of low-dose and high- dose,the cell OD value,survival rate and the number of colony formation were decreased,and the apoptosis rate, G1 period as well as expression levels of mRNA and protein of NCR1 and NKp46 were increased(P<0.05). Compared with those in the cisplatin group,in the low- dose group,the cell OD value,survival rate and the number of colony formation were increased,and the apoptosis rate,G1 period as well as expression levels of mRNA and protein of NCR1 and NKp46 were decreased(P<0.05);in the high-dose group,the cell OD value,survival rate and the number of colony formation were decreased,and the apoptosis rate,G1 period as well as expression levels of mRNA and protein of NCR1 and NKp46 were increased(P<0.05). Compared with those in the low-dose group,the cell OD value,survival rate and the number of colony formation in the high-dose group were decreased(P<0.05),and the apoptosis rate,G1 period as well as expression levels of mRNA and protein of NCR1 and NKp46 were increased(P<0.05). Conclusion: Shenqi Fuzheng injection can inhibit the proliferation of human lung cancer LEWIS,promote the apoptosis of LEWIS. Its mechanism is that Shenqi Fuzheng injection can promote the mRNA and protein expression of NCR1 and NKp46 in the lung cancer LEWIS cell,and then mediate NK cells to kill the lung cancer cells.