Abstract：Objective： To observe the inhibitory effect on gastric cancer cells through cryptotanshinone regulating Wnt/β- catenin signaling pathway. Methods： Human gastric cancer BGC- 823 cells were divided into three groups： the control group，the low-dose cryptotanshinone group，the medium-dose cryptotanshinone group，the high-dose cryptotanshinone group(shorthand for the low，medium and high dose groups)，the high-dose +SKL2001 group and the high-dose +XAV939 group. The low，medium and high dose groups were applied with 20，40 and 80 μmol/L cryptotanshinone during the culture process；the high-dose +SKL2001 group was added with 80 μmol/L cryptotanshinone and 20 μmol/L SKL2001；the highdose +XAV939 group was added with 80 μmol/L cryptotanshinone and 1 μmol/L XAV939. The MTT assay，Transwell assay and cell scratch assay were used to detect the proliferation，migration and invasion of cells. The RT-PCR and protein western blot were used to detect the expression levels of Dishevelled-2(Dvl2)，Glycogen Synthase Kinase-3β(GSK-3β)，β-catenin and Cyclin D1 in cells. Results：Compared with those in the control group，the cell proliferation inhibition rate，levels of GSK- 3β protein and mRNA expression were increased in the the low， medium and high dose groups 24， 48 and 72 h after intervention(P＜0.05)， and the levels of Dvl2， β- catenin， Cyclin D1 protein and mRNA expression were decreased(P＜ 0.05)， and the dose- dependent feature was shown(P＜0.05). Forty- eight hours after cell scratch， the migration ability of cells，the number of transmembrane cells and the healing rate of the scratch were all decreased in the the low，medium and high dose groups(P＜0.05)， and they decreased as the dose increased(P＜0.05). Compared with those in the high dose group， the migration ability， the number of transmembrane cells and the healing rate of the scratch in the high- dose + SKL2001 group were all increased(P＜0.05)；compared with those in the high-dose +SKL2001 group，the above indexes in the high-dose +XAV939 group were all decreased(P＜0.05). Conclusion：Cryptotanshinone can inhibit gastric cancer cells by inhibiting the activity of Wnt/β-catenin signaling pathway.