Abstract： Objective： To study the contents of mineral elements and flavonoids in eleutherine plicata herb and its antioxidant activity. Methods：ICP- MS method was applied to determine the contents of mineral elements in eleutherine plicata herb，which included Mg，Fe，B，Cr，Mn，As，Cu，Pb，Se，Zn，Hg and Ca. NaNO2-Al(NO3)3 was extracted by ultrasound. The content of total flavonoids was analyzed by color- developing method. Antioxidant activity of eleutherine plicata herb was analyzed from DPPH· scavenging rate，·OH scavenging rate，and total reducing power. Results：In the samples of eleutherine plicata herb，there were abundant selected mineral elements with different contents，but Zn，Hg and Ca were not detected. The extraction rate of total flavonoids of eleutherine plicata herb was 1.97%，with good DPPH· scavenging rate and ·OH scavenging rate；the scavenging rate and total reducing power were positively correlated with the concentration of total flavonoids. Conclusion： Eleutherine plicata herb has rich mineral elements and good antioxidant activity， which can provide a theoretical basis for the subsequent development of food additives， cosmetics， health products and so on.