Abstract：Objective：To observe the clinical effect of Bushen Ningxin prescription for early-onset ovarian insufficiency of non- interaction between the heart and kidney type. Methods： A total of 60 cases of patients with early- onset ovarian insufficiency of non-interaction between the heart and kidney type were selected and divided into the control group and the observation group according to the random number table method，30 cases in each group. The control group was treated with Femoston，and the observation group was given Bushen Ningxin prescription. Both groups were treated for three months. Before and after treatment， Kupperman scores， Chinese medicine syndrome scores， follicle stimulating hormone(FSH)， luteinizing hormone(LH)，estradiol(E2)，anti-müllerian hormone(AMH)，5-hydroxytryptamine(5-HT)，ovarian volume，the number of antral follicles，endometrial thickness，and the resistance index(RI) of ovarian artery were compared between two groups. Results：Before treatment，there were no significant differences being found in the comparison of Kupperman score and Chinese medicine syndrome score between the two groups(P＞0.05). After treatment， the Kupperman scores and Chinese medicine syndrome scores in the two groups were decreased when compared with those before treatment (P ＜ 0.05)，and the two scores above in the observation group were lower than those in the control group(P＜0.05). Before treatment，there were no significant differences being found in the comparison of the levels of FSH，LH，E2，AMH，and 5- HT between the two groups(P＞0.05). After treatment，the levels of FSH and LH in the two groups were decreased when compared with those before treatment，and the levels of E2 and AMH were increased(P＜0.05)；the level of 5- HT in the observation group was increased when compared with that before treatment(P＜0.05). After treatment，the levels of FSH and LH in the observation group were lower than those in the control group，and the level of 5-HT was higher(P＜0.05). Before treatment，when compared the ovarian volume，the number of antral follicles，endometrial thickness，and RI between the two groups， there were no significance being found in differences(P＞0.05). After treatment， the ovarian volume， the number of antral follicles，and endometrial thickness in the two groups were increased when compared with those before treatment(P＜0.05). Conclusion：For patients with early-onset ovarian insufficiency of non-interaction between the heart and kidney type，Bushen Ningxin prescription can improve their clinical symptoms，the levels of hormone，and ovarian reserve function.