Abstract：Objective：To observe the clinical effect of Shenqi Fuzheng injection combined with chemotherapy for nonsmall cell lung cancer(NSCLC). Methods：A total of 80 cases of NSCLC patients were randomly divided into two groups， 40 cases in each group. The control group was given GP chemotherapy，and the combination group was additionally treated with Shenqi Fuzheng injection based on the treatment of the control group. Before and after treatment，the changes in the levels of serum tumor markers including carcinoembryonic antigen(CEA)，carbohydrate antigen(CA199) and neuron specific enolase(NSE) as well as T cell subsets including CD4 + and CD4 +/CD8 + in the two groups were observed and compared. The recent curative effect and adverse reactions in the two groups were observed and compared. Results：The total effective rate was 60.0% in the combination group and 37.5% in the control group， the difference being significant(P＜0.05). After treatment，the levels of CEA，CA199 and NSE in serum in the two groups were significantly decreased when compared with those before treatment(P＜0.05)，and all the levels in the combination group were lower than those in the control group (P＜0.05). After treatment，CD4+ and CD4+/CD8+ in the two groups were significantly increased when compared with those before treatment(P＜0.05)，and the improvement of the two above indexes in the combination group was more significant than that in the control group(P＜0.05). During treatment，the incidence of abnormal blood routine，digestive tract reaction， fatigue and other adverse reactions in the combination group was lower than that in the control group，differences being significant(P＜0.05). Conclusion：The therapy of Shenqi Fuzheng injection combined with GP chemotherapy for NSCLC can not only increase the short- term clinical total effective rate and reduce the adverse reactions，but also reduce the levels of serum tumor markers and enhance the immune function.