Abstract： Objective： To observe the clinical effect of moxibustion at governor vessel combined with Bushen Quhan Huashi prescription for ankylosing spondylitis(AS) and its effect on levels of C-terminal telopeptide of typeⅠcollagen(CTX-I) and Dickkopf-1 protein(DKK1) in serum. Methods：A total of 160 cases of AS patients were selected and divided into group A，group B，group C and group D by envelope method，40 cases in each group. Group A was given routine symptomatic treatment of western medicine；group B was additionally treated with moxibustion at governor vessel based on the treatment of group A； group C was additionally treated with Bushen Quhan Huashi prescription based on the treatment of group A； group D was additionally treated with moxibustion at governor vessel and Bushen Quhan Huashi prescription based on the treatment of group A. All groups were treated for three months. The Chinese medicine syndrom score was compared among the four groups. The improvement of symptoms before and after treatment as well as levels of erythrocyte sedimentation rate(ESR)， C- reactive protein(CRP)， CTX- I and DKK1 were compared among the four groups. Results： After treatment， the Chinese medicine syndrome scores in the four groups were decreased when compared with those before treatment(P＜ 0.05)；and the groups with the scores from low to high were group D，group C，group B and group A；the score in group D was significantly lower than those in group A，group B and group C(P＜0.05). After treatment，thoracic mobility in the four groups were increased when compared with those before treatment(P＜0.05)；and the groups with the thoracic mobility from large to small were group D，group C，group B and group A；occiput-to-wall distance and finger-to-floor distance in the four groups were decreased when compared with those before treatment(P＜0.05). Bath Ankylosing Spondylitis Disease Activity Index(BASDAI) and Bath Ankylosing Spondylitis Functional Index(BASFI) in the four groups were decreased when compared with those before treatment(P＜0.05). After treatment， occiput- to- wall distance， finger- to- floor distance， BASDAI and BASFI in group D were lower than those in group A，group B and group C(P＜0.05)，and the four indexes in group B and group C were lower than those in group A(P＜0.05). After treatment，levels of ESR，CRP，CTX-I and DKK1 in the four groups were decreased when compared with those before treatment(P＜0.05)；the four levels in group D were lower than those in group A，group B and group C(P＜0.05)，and the four levels in group B and group C were lower than those in group A(P＜0.05). Conclusion： The therapy of moxibustion at governor vessel combined with Bushen Quhan Huashi prescription can relieve inflammatory reactions of AS patients， regulate the expression of CTX- I and DKK1， and improve body activity function.