Abstract：Objective：To discuss the effect of medicated serum with Radix et rhizoma notoginseng on proliferation and migration of fibroblast L929 as well as expression of vascular endothelial growth factor(VEGF)，transforming growth factor-β (TGF-β)，and typeⅠcollagen(CollagenⅠ). Methods：A total of 28 cases of SD rats of SPF level were randomly divided into the control group and the Radix et rhizoma notoginseng groups of low，medium and high dose and the serum was prepared. Fibroblast line L929 of mice was cultured in vitro；the control group was given serum with normal saline，and the groups of low，medium and high dose were given corresponding intervention of medicated serum with Radix et rhizoma notoginseng. The CCK- 8 method was applied to detecting proliferation of fibroblast；scratch assay was applied to detecting migration of fibroblast；RT-qPCR and Western Blot was applied to detecting the expression of mRNA and protein of VEGF，TGF-β and Collagen Ⅰrespectivehy. Results： Compared with those in the control group， in the low- dose group， the OD value 24 h after intervention and migration healing percentage 48 h after intervention were increased(P＜0.05)， and the expression of mRNA and protein of VEGF and TGF- β was increased(P＜0.05)； in the medium- dose group， the OD value 24 h after intervention and migration healing percentage 48 h after intervention were increased(P＜0.05)，and the expression of mRNA and protein of VEGF，TGF- β，and Collagen Ⅰwas increased(P＜0.05)；in the high- dose group，the OD value 24 h after intervention and migration healing percentage 48 h after intervention were decreased(P＜0.05)，and the expression of mRNA and protein of VEGF and TGF-β was decreased(P＜0.05). Compared with those in the low-dose group，in the medium-dose group， the OD value 24 h after intervention and migration healing percentage 48 h after intervention were increased(P＜ 0.05)，and the expression of mRNA and protein of VEGF，TGF-β，and CollagenⅠwas increased(P＜0.05)；in the high-dose group， the OD value 24 h after intervention and migration healing percentage 48 h after intervention were decreased(P＜ 0.05)，and the expression of mRNA and protein of VEGF and TGF- β was decreased(P＜0.05). Compared with those in the medium-dose group，in the high-dose group，the OD value 24 h after intervention and migration healing percentage 48 h after intervention were decreased(P＜0.05)，and the expression of mRNA and protein of VEGF，TGF-β，and CollagenⅠwas decreased(P ＜ 0.05). Conclusion ： Medium-dose medicated serum with Radix et rhizoma notoginseng can promote proliferation and migration of fibroblast，and up-regulate the expression of VEGF，TGF-β，and CollagenⅠ，whose curative effect is better than that of low- dose therapy. High- dose medicated serum with Radix et rhizoma notoginseng inhibits proliferation and migration of fibroblast as well as the expression of VEGF and TGF- β，which is not conducive to healing of wound.