Abstract：Objective：To observe the clinical effect of tuina at different interval time combined with positioning rotating and pulling manipulation at cervical spine for cervical spondylosis of the neck type， and to discuss the best interval time between three- step tuina manipulation and intervention of positioning rotating and pulling manipulation at cervical spine. Methods：A randomized controlled research method with a 2×2 factorial design was adopted. The design scheme included factors of treatment interval time(treatment for once every two days or every four days)，and factors of positioning rotating and pulling manipulation at cervical spine(application or without application). A total of 60 cases of patients with cervical spondylosis of the neck type were randomly divided into the group with tuina every two days+positioning rotating and pulling manipulation at cervical spine，the group with tuina every two days+no positioning rotating and pulling manipulation at cervical spine position)，the group with tuina every four days+ positioning rotating and pulling manipulation at cervical spine，and the group with tuina every four days +no positioning rotating and pulling manipulation at cervical spine，15 cases in each group. The clinical effect was compared among the four groups. The change in soft tissue tension was observed in the four groups before and after treatment. Main effect and interaction effect of two factors were analyzed. Results：The total effective rate was 93.3% in the group with tuina every two days and rotating and pulling manipulation，86.7% in the group with tuina every two days and without rotating and pulling manipulation，86.7% in the group with tuina every four days and rotating and pulling manipulation，and 80.0% in the group with tuina every four days and without rotating and pulling manipulation，there being no significance in the difference(P＞0.05). After treatment， soft tissue tension in the four groups was increased when compared with that before treatment(P＜0.01). The comparison of difference values of soft tissue tension before and after treatment among the four groups showed significance in the difference(P＜0.01). The difference value of soft tissue tension before and after treatment in the group of with tuina every two days and rotating and pulling manipulation was higher than those in the other three groups(P＜0.01)；the difference value of soft tissue tension in the group with tuina every four days and without rotating and pulling manipulation was lower than those in the other three groups(P＜0.01). There were two factors：main effect of factors of treatment interval(P＜0.01)，and main effect of positioning rotating and pulling manipulation at cervical spine(P＜0.01)， there was no interaction effect between the two factors above(P＞0.05). Conclusion： In the treatment of cervical spondylosis of the neck type，there were two important factors related with curative effect，namely， the interval time of tuina and the usage of positioning rotating and pulling manipulation at cervical spine，As for improvement of soft tissue tension，the therapy of tuina every two days and positioning rotating and pulling manipulation at cervical spine has the best effect.