Abstract：Objective：To study protective effect of oxidative damage model of ARPE-19 cells and naringenin phospholipid complex(NPC) on ARPE- 19 cells with oxidative damage. Methods： According to the best preparation process recorded on literature， NPC was prepared. The solubility of NPC in water was determined. According to the effect of different concentrations of Tert-butyl hydroperoxlde(T-BHP) on the survival rate of ARPE-19 cells，the best oxidative damage model was selected. ARPE- 19 cells were divided into the solvent group， the model group， the naringenin groups of lowdose， medium-dose and high-dose，the NPC groups of low-dose，medium-dose and high-dose. The solvent group was cultured with dimethyl sulfoxide(DMSO) ； the other group was intervened with 200 μmol/L T-BHP；the naringenin groups of low- dose， medium- dose and high- dose were intervened with 100 μmol/L， 200 μmol/L and 400 μmol/L naringenin respectively；the NPC groups of low- dose，medium- dose group and high- dose were intervened with NPC of 100 μmol/L， 200 μmol/L and 400 μmol/L， respectively. MTT method was used to detect the cell survival rate， and flow cytometry was used to detect the apoptosis rate. Results： Compared with naringenin， the solubility of naringenin phospholipid mixture and NPC in water was increased(P＜0.01). Compared with naringenin phospholipid mixture， the solubility of NPC in water was increased(P＜0.01). When the concentration of T- BHP was 200 μmol/L，the survival rate of ARPE-19 cells was 50%，which was the best oxidative damage model. Compared with the solvent group，the survival rate of ARPE-19 cells in the model group was significantly decreased(P＜0.01)，and the apoptosis rate was significantly increased (P＜0.01). Compared with the model group，the survival rate in the naringenin groups of medium-dose and high-dose as well as the NPC groups of low- dose， medium- dose and high- dose was increased(P＜0.05)， and the apoptosis rate was decreased(P＜0.01). Compared with the low-dose NPC group，the cell survival rate in the NPC groups of medium-dose and high- dose was increased(P＜0.05)， and the apoptosis rate was decreased(P＜0.01). Compared with the low- dose NPC group，the apoptosis rate in the naringenin groups of low-dose and medium-dose was increased(P＜0.01). Compared with the medium-dose NPC group，the survival rate in the naringenin groups of low-dose，medium-dose and high-dose as well as the low- dose NPC group was decreased(P＜0.05)， and the apoptosis rate was increased(P＜0.01). Compared with the high-dose NPC group，the survival rate in the naringenin groups of low-dose，medium-dose and high-dose as well as the low- dose NPC group was decreased(P＜0.05)， and the apoptosis rate was increased(P＜0.01). Conclusion： When the phospholipid complex of naringenin forms， the water solubility and biological activity of naringenin increases， and its protective effect on ARPE-19 cells with oxidative damage is significantly enhanced.