Abstract：Objective：To observe the effect of Jiangtang Yishen tang combined with alprostadil on intact fibroblast growth factor- 23(iFGF- 23)， α- Klotho and ubiquitin- ribosomal binding protein 52(UBA5) in patients with diabetic nephropathy. Methods：A total of 118 cases of patients with diabetic nephropathy were selected as the study objects，and were divided into the control group and the observation group according to the random number table method，59 cases in each group. The control group was treated with alprostadil， and the observation group was additionally treated with Jiangtang Yishen tang based on the treatment of the control group. The changes of serum iFGF-23，α-Klotho，UBA52，creatinine(Cr)，blood urea nitrogen(BUN)， total cholesterol(TC)， triacylglycerol(TG)， creatinine clearance rate(CCr)， urinary albumin excretion rate (UAER) before and after treatment as well as the clinical effect in the two groups were compared. Results：The total effective rate was 94.92% in the observation group， significantly higher than that of 83.05% in the control group(P＜0.05). After treatment，serum iFGF-23，UBA52，BUN，Cr，TC，TG and UAER in the two groups were all decreased when compared with those before treatment(P＜0.05)， and α- Klotho and CCr were increased(P＜0.05). After treatment， serum iFGF- 23， UBA52，BUN，Cr，TC，TG and UAER in the observation group were all decreased when compared with those in the control group(P＜0.05)， and α- Klotho and CCr were increased(P＜0.05). Conclusion： The therapy of Jiangtang Yishen tang combined with alprostadil can regulate the levels of iFGF- 23 ， α - Klotho and UBA52 in patients with diabetic nephropathy，protect kidney function，and correct metabolic disorders，whose curative effect is better than that of simple alprostadil.