Abstract：Objective：To observe the effect of matrine combined with lamivudine for chronic hepatitis B and its effect on liver fibrosis indexes. Methods：A total of 88 cases of patients with chronic hepatitis B were divided into the control group and the observation group according to the random number table method， 44 cases in each group. Both groups were given routine treatment. The control group was additionally given oral administration of lamivudine tablets；based on the treatment of the control group，the observation group was additionally given intravenous infusion of matrine injection for three months and oral administration of matrine capsules for nine months. Both groups were treated for 12 months. The curative effect was observed，and the effect on liver fibrosis indexes was compared and analyzed. Results：After treatment，the liver fibrosis indexes including laminin(LN)， hyaluronic acid(HA)， procollage Ⅲ(PC Ⅲ) and collagen Ⅳ( Ⅳ- C) in the control group were decreased，and the above indexes in the observation group were decreased and lower than those in the control group(P＜ 0.05). After treatment， the recovery rate of alanine aminotransferase(ALT)， the conversion rate of hepatitis B e antiagen (HBeAg)，and the negative conversion rates of HBV-DNA and hepatitis B e antigen(HBeAg) in the observation group were all higher than those in the control group(P＜0.05). The total effective rate in the observation group was higher than that in the control group(χ 2=4.768，P=0.029＜0.05). Conclusion：The therapy of matrine combined with lamivudine for chronic hepatitis B can effectively reduce liver fibrosis degree，which is conducive to the recovery of liver function with good curative effect.