Abstract：Objective：To evaluate the protective effect of Aikang tablets on cyclophosphamide- induced cardiotoxicity. Methods：A total of 40 BALB/c- nu mice were randomly divided into the normal group，the model group，and the Aikang tablets groups of high- dose，medium- dose，and low- dose，8 mice in each group. Except for the normal group，in the other groups， cyclophosphamide- induced cardiotoxicity models were established by intraperitoneal injection of 0.3 g/kg cyclophosphamide for continuous two weeks，once a week. On the day of model establishment，the Aikang tablets groups of high- dose，medium- dose，and low- dose were given gastric perfusion of 0.046 g/mL，0.023 g/mL，and 0.012 g/mL solution of Aikang tablets respectively at the dose of 20 mL/(kg·d)； the Aikang tablets groups received two weeks of continuous medication，once a day. The normal group and the model group were given gastric perfusion of distilled water of the same volume at the dose of 20 mL/(kg·d). The content of lactic dehydrogenase(LDH)，hydroxybutyric dehydrogenase (HBDH)， aspartate aminotransferase(AST)， creatine kinase(CK)， and creatine kinase isoenzyme(CKMB) in serum were detected in each group. Cardiac index was calculated. The change in cardiopathology was observed through HE staining. Results：Compared with those in the normal group，in the model group，LDH and HBDH in serum as well as cardiac index were increased(P＜0.05)；rank values of cardiomyocyte hypertrophy，cardiomyocyte degeneration，and inflammatory cell infiltration as well as individual score were increased(P＜0.01). Compared with those in the model group，in the Aikang tablets groups of high-dose，medium-dose，and low-dose，LDH and HBDH in serum as well as cardiac index were decreased(P＜ 0.01). Compared with those in the model group，rank value of cardiomyocyte hypertrophy and individual score in the highdose Aikang tablets group were decreased(P＜0.05)； rank values of cardiomyocyte hypertrophy， cardiomyocyte degeneration，and inflammatory cell infiltration as well as individual score were decreased in the medium-dose Aikang tablets group(P＜0.05，P＜0.01). Conclusion：Aikang tablets has the protective effect on cyclophosphamide-induced cardiotoxicity.