Abstract：Objective：To observe the clinical effect of Kanglaite Injection for patients with non-small cell lung cancer after chemotherapy. Methods：A total of 98 cases of patients with non-small cell lung cancer were divided into the control group and the observation group according to the random number table method，49 cases in each group. The control group was treated with GP chemotherapy scheme， and the observation group was additionally treated with Kanglaite injection based on the treatment of the control group. Both groups were treated for two treatment cycles，21 days being one cycle. The clinical effect in the short term was compared between the two groups. The comparison of the changes in immune function evaluated by levels of CD8 + ， CD4 + ， CD3 + and CD4 +/CD8 + ， hematological toxicity evaluated by levels of blood platelet count(BPC)， hemoglobin(Hb) and white blood cell(WBC)， tissue inhibitors of metalloproteinase- 1(TIMP- 1)， and matrix metalloprotein 9(MMP-9) in serum between the two groups was carried out. Results：The effective control rate and total effective rate was 83.67% and 36.73% in the observation group，and 77.55% and 32.65% in the control group，there being no significance in differences(P＞0.05). After treatment，the levels of CD8+，CD4+，CD3+ and CD4+/CD8+ in the two groups were decreased when compared with those before treatment(P＜0.05)；but the levels of CD8+，CD4+ and CD3+ in the observation group were higher than those in the control group(P＜0.05)；there was no significant difference being found in the comparison of CD4 +/CD8 + between the two groups(P＞0.05). After treatment， MMP- 9 in the two groups was decreased when compared with that before treatment(P＜0.05)，and the content of MMP-9 in the observation group was lower than that in the control group(P＜0.05). There were little changes in levels of TIMP- 1 in the two groups before and after treatment(P＞0.05). The curative effect of BPC，Hb，and WBC in terms of the toxicity grading in the observation group was better than that in the control group，the difference being significant(P＜0.05). Conclusion：Kanglaite Injection can improve the immune function of patients with non- small cell lung cancer undergoing chemotherapy， and reduce the effect of hematological toxicity，with significant clinical effect.