Abstract：Objective：To observe the clinical effect of Chaihu Guizhi tang for patients with chronic fatigue syndrome(CFS). Methods：A total of 130 cases of CFS patients were selected as the study objects and divided into the observation group and the control group according to different methods，65 cases in each group. The control group was treated with acupuncture， and the observation group was additionally treated with Chaihu Guizhi tang based on the treatment of the control group. Observe the clinical effect of the two groups. Results： The total effective rate was 92.3% in the observation group， and 76.9% in the control group， there being significant difference in the comparison of total effective rates between the two groups(P＜0.05). When the two groups were enrolled，the scores of mental fatigue and physical fatigue as well as the total scores of Fatigue Scale-14(FS-14) in the two groups were similar，there being no significant difference(P＞0.05). At the end of the treatment， the scores in the two groups were improved， and there was significant difference being found in the comparison of the scores of mental fatigue and physical fatigue as well as the total scores of FS-14 between the two groups (P＜0.05). At the time of enrollment， the levels of IgA， IgG and IgM in the two groups were similar， there being no significant difference(P＞0.05). At the end of the treatment， the immunoglobulin levels in the observation group were improved， and the levels of IgA， IgG and IgM in the observation group were higher than those in the control group， the difference being significant(P＜0.05). Conclusion：The application of Chaihu Guizhi tang for CFS patients has reliable clinical effect，which can enhance the immunity and improve the fatigue degree of patients.