Abstract： Objective： To observe the effect of Xiezhuo Tongluo method on immune function in patients with chronic kidney disease(CKD) at stage 3 to 5. Methods：A total of 84 cases of patients with CKD at stage 3 to 5 were selected and divided into the control group and the treatment group according to the random number table method， 42 cases in each group；a total of 20 healthy volunteers were recruited as the normal group. The control group was given routine symptomatic treatment， and the treatment group was additionally treated with Xiezhuo Tongluo method based on the treatment of the control group； both groups were treated for three months. The normal group was not given treatment. Before and after treatment，the levels of CD4+ and CD8+，the values of CD4+/CD8+，and the levels of interleukin-2(IL-2)，soluble interleukin-2 receptor(sIL-2R)，immunoglobulin(Ig) G，IgA and IgM in serum，red blood cell-C3b receptor rosette rate(RBC-C3bRR) and red blood cell- immune complex rosette rate(RBC- ICR) in the three groups were compared. Results： Before treatment， CD4+ levels and CD4+/CD8+ values in the control group and the treatment group were lower than those in the normal group(P＜ 0.05)，and CD8+ levels were higher(P＜0.05). After treatment，CD4+ level and CD4+/CD8+ value in the treatment group were increased when compared with those before treatment(P＜0.05)，and CD8 + level was decreased(P＜0.05)；CD4 + level and CD4+/CD8+ value in the treatment group were higher than those in the control group(P＜0.05)，and CD8+ level was lower(P＜ 0.05). Before treatment，IL-2 levels in the control group and the treatment group were lower than those in the normal group (P＜0.05)，and sIL-2R levels were higher(P＜0.05). After treatment，IL-2 level in the treatment group was increased when compared with that before treatment(P＜0.05)，and sIL-2R level was decreased(P＜0.05)；IL-2 level in the treatment group was higher than that in the control group(P＜0.05)， and sIL- 2R level was lower(P＜0.05). Before treatment， the levels of IgG，IgA and IgM in the control group and the treatment group were lower than those in the normal group(P＜0.05). After treatment，the levels of IgG，IgA and IgM in the treatment group and IgG level in the control group were increased when compared with those before treatment(P＜0.05)； the levels of IgG， IgA and IgM in the treatment group were higher than those in the control group(P＜0.05). Before treatment，the levels of RBC-C3bRR in the control group and the treatment group were lower than that in the normal group(P＜0.05)，and the levels of RBC- ICR were higher(P＜0.05). After treatment，the level of RBC- C3bRR in the treatment group was increased when compared with that before treatment(P＜0.05)， and the level of RBC- ICR was decreased(P＜0.05)； the level of RBC- C3bRR in the treatment group was higher than that in the control group(P＜0.05)，and the level of RBC- ICR was lower(P＜0.05). Conclusion：Xiezhuo Tongluo method can improve the immune function of cells，body fluids and red blood cells in patients with CKD at stage 3 to 5.