Abstract：Objective：To observe the clinical effect of cupping following collateral bloodletting for heat stroke. Methods： A total of 603 cases of patients with heat stroke were divided into the control group and the observation group， 286 and 317 cases in each group respectively. The control group was given routine treatment for decreasing body temperature，and the observation group was treated with cupping following collateral bloodletting. The cure rate， the incidence of adverse reactions，the myocardial markers levels，the hospitalization time and the hospitalization expenses in the two groups were compared. Results：The cure rate was 87.07% in the observation group，higher than that of 79.02% in the control group(P＜ 0.05). There was no significant adverse reactions in the observation group，and the incidence of adverse reactions was 2.10% in the control group， the difference being significant(P＜0.05). After treatment， the levels of cardiac troponin I(cTnI) and creatine kinase muscle- brain(CK- MB) in serum in the two groups were decreased when compared with those before treatment(P＜0.05)，and the levels of cTnI and CK-MB in serum in the observation group were lower than those in the control group(P＜0.05). Conclusion：Cupping following collateral bloodletting for heat stroke can significantly improve the cure rate， decrease myocardial markers levels，which is safe and effective.