Abstract：Objective：To observe the effect of diammonium glycyrrhizinate combined with thymosin α1(Tα1) on levels of serum cholinesterase(CHE) and total bile acids(TBA) in patients with viral hepatitis. Methods：A total of 110 patients with viral hepatitis were selected and divided into the control group and the combination group according to stratified sampling method， 55 cases in each group. The control group was treated with Tα1， and the combination group was additionally treated with diammonium glycyrrhizinate based on the treatment of the control group. The levels of total cholesterol(TC)，triglyceride(TG)， high density lipoprotein cholesterol(HDL- C)， low density lipoprotein cholesterol(LDL- C)， CD3 + ， CD4 + ， CD8 + ， albumin (Alb)， aspartate aminotransferase(AST)， alanine aminotransferase(ALT)， total bilirubin(TBil)， TBA， procollagen Ⅲ(PC Ⅲ)， Type Ⅳ collagen(Ⅳ-C)，laminin(LN) and CHE before and after treatment in the two groups were analyzed. The value of CD4+ over CD8+ was calculated. The clinical effect and the incidence of adverse reactions were compared between the two groups. Results：Compared with those before treatment，the levels of TC，TG，HDL-C，LDL-C，CD8+，AST，ALT，TBil，PCⅢ， Ⅳ-C，LN and TBA were all decreased in both groups after treatment(P＜0.05)；the levels of CD3+，CD4+，Alb，CHE and CD4+/CD8+ were increased(P＜0.05). the levels of TC，TG，HDL-C，LDL-C，CD8+，AST，ALT，TBil，PCⅢ，Ⅳ-C，LN and TBA in the combination group were lower than those in the control group(P＜0.05)，and the levels of CD3+，CD4+，Alb， CHE and CD4+/CD8+ were higher(P＜0.05). The total effective rate was 98.18% in the combination group，higher than that of 85.45% in the control group(P＜0.05). There was no significant difference in the incidence of adverse reactions between the two groups(P＞0.05). Conclusion： Diammonium glycyrrhizinate combined with Tα1 can effectively alleviate the clinical symptoms of patients with viral hepatitis，increase CHE level，reduce TBA level and improve liver function，which has a antiliver fibrosis effect.