Abstract：Objective：To investigate the effect of baicalin on the proliferation and invasion of human prostate cancer cells (PC3) and its mechanism. Methods：CCK-8 was used to detect the effect of baicalin on PC3 proliferation；Transwell test was used to detect the effect of baicalin on PC3 cell invasion ability， and Western blot was used to detect the expression of E-cadherin and pro-apoptotic genes Bax and caspase-3，anti-apoptotic gene Bcl-XL and autophagy-related genes Beclin-1 and LC3B-Ⅱ of PC3 treated with baicalin. Results：Baicalin could inhibit the proliferation and invasion of PC3，and there was a concentration-dependent trend. Baicalin could promote the expression of E-cadherin in PC3. Baicalin could up-regulate the expression of pro- apoptotic genes Bax and caspase- 3， and inhibit the expression of anti- apoptotic gene Bcl- XL in PC. Baicalin could promote the expression of Beclin- 1 and LC3B- Ⅱ in PC3. Conclusion： Baicalin can promote apoptosis and autophagy，increase the expression of E-cadherin，and inhibit the proliferation and invasion ability of PC3.