Abstract： Objective： To observe the effect of vitex gluconyl flavonoids on inhibiting autophagy- induced apoptosis of non- small cell lung cancer A549 cells and its effect on survival of nude mice with transplanted tumor. Methods： In vitro experiment，different concentrations(10 μmol/L，20 μmol/L，40 μmol/L) of vitex gluconyl flavonoids were used to treat lung cancer A549 cells. Cell proliferation was detected by CCK8 assay；cell apoptosis was detected by flow cytometry；Western Blotting was used to detect the protein expressions of Ki67，PCNA，Caspase-3，Caspase-9，P62，Beclin1，LC3Ⅱ/LC3Ⅰ， ATG5 and the phosphorylation of JNK1/2 and ERK1/2. In vivo experiment，the transplanted tumor models were established by subcutaneous injection of A549 cells， and they were intraperitoneal injected with 0.5 mg/kg， 1 mg/kg， 2 mg/kg Vitex gluconoids，respectively. The survival curve was plotted on Day 30，and the expression of Ki67 and Caspase- 3 in tissues was detected by immunohistochemistry. Results：Vitex gluconyl flavonoids could inhibit the survival of A549 cells，decrease the expression of Ki67 and PCNA proteins，promote cell apoptosis and expression of Caspase- 3 and Caspase- 9 proteins， down-regulate utophagy markers Beclin1，LC3Ⅱ/LC3Ⅰ and ATG5，up-regulate P62 protein expression，increase JNK1/2 and decrease the phosphorylation level of ERK1/2. It also increased the survival rate，down- regulated Ki67 expression and up- regulated the expression of Caspase- 3 in nude mice with transplanted tumor. Conclusion： Vitex glucomylflavone can induce apoptosis of A549 cells by inhibiting autophagy，and increase the survival rate of nude mice with transplanted tumor.