Abstract： Objective： To observe the effect of Wenjing Tang on the prevention and treatment of athletic menstrual irregularities rats. Methods： A total of 30 rats were randomly divided into blank group， model group， and Wenjing Tang groups(10 rats in each group). A rat model of athletic menstrual irregularities in aquatic sports was reproduced by exhaustive swimming exercise.Wenjing tang group was given Wenjing Tang gavage，and blank group，model group were given distilled water for a total of 4 weeks.The levels of progesterone(P)，estradiol(E2)，testosterone(T)，luteinizing hormone(LH)，follicle stimulating hormone(FSH) were measured by enzyme linked immunosorbent assay(ELISA) in rats. Results：When compared with the blank group，the FSH content of rats in the model group decreased and the difference was statistically significant(P＜ 0.05)， and the FSH content of rats in the Wenjing Tang group was slightly higher than that of the blank group， but the difference was not statistically significant(P＞0.05). Compared with the model group， FSH of rats in Wenjing Tang group increased significantly(P＜0.05). Although there was no significant difference in LH content among groups(P＞0.05)， only numerically，the LH content of rats in Wenjing Tang group was the highest，while that of rats in model group was the lowest. Compared with the blank group，the P content of rats in the model group was significantly reduced and the difference was statistically significant(P＜0.01)，while the P content of rats in the Wenjing Tang group was slightly reduced and the difference was not statistically significant(P＞0.05). Compared with the model group，the rats in Wenjing Tang group had a significant rise in P，and the difference was statistically significant(P＜0.05).Compared with the blank group，the level of T decreased in rats with model group，the difference was statistically significant(P＜0.05)，and it decreased slightly in Wenjing Tang group (P＞0.05). Compared with the model group，the T content of rats in Wenjing Tang was numerically higher，but the difference was not statistically significant(P＞0.05).Compared with the blank group， the E2 content of rats in the model group group significantly decreased，the difference was statistically significant(P＜0.01)，the E2 content of rats in the Wenjing Tang group decreased， the difference was not statistically significant(P＞0.05). Conclusion： Wenjing Tang has a preventive effect on athletic menstrual irregularities.