Abstract：Objective：To observe the effect of electroacupuncture at Tianshu point(ST 25) and Shangjuxu point(ST 37) on the expression of vitamin D receptor(VDR) of intestinal mucosa of rats with colitis，and to discuss the possible mechanism of electroacupuncture in the treatment of ulcerative colitis(UC). Methods：Male Wistar rats were divided into the control group， the model group and the electroacupuncture group according to the random number table method，eight rats in each group. The rat models of UC were induced by enema with trinitrobenzene sulfonic acid(TNBS). Meanwhile，Disease Activity Index (DAI) and Histology Activity Index(HAI) were used to evaluate the degree of colitis. In the electroacupuncture group，rats were given electroacupuncture at Tianshu point(ST 25) and Shangjuxu point(ST 37) for 14 days， started from the next day after successful TNBS modeling. The colonic tissues were collected after the rats were sacrificed on the 15th day. The expression level of VDR of intestinal mucosa was measured by immunohistochemistry and real-time quantitative reverse transcription- PCR(qRT- PCR). Myeloperoxidase(MPO) activity in colonic tissue was measured by spectrophotometry. Results： When compared with that in the model group， HAI in the electroacupuncture group was obviously decreased(P=0.005). When compared with that in the control group，the activity of MPO in colonic tissue in the model group and the electroacupuncture group were significantly increased. The activity of MPO in colonic tissue in the electroacupuncture group was decreased when compared with that in the model group(P＜0.001). The expression of mRNA and protein of VDR in intestinal mucosa in the model group and the electroacupuncture group was decreased when compared with that in the control group(P＜0.01). The expression of mRNA and protein of VDR in intestinal mucosa in the electroacupuncture group was decreased when compared with that in the model group(P＜0.05). Protein immunohistochemistry suggested that VDR was mainly expressed in epithelial cells. The expression level of protein of VDR inversely correlated with MPO activity(P＜0.05). Conclusion： The electroacupuncture at Tianshu point(ST 25) and Shangjuxu point(ST 37) may help relieve UC of rats by up- regulating the expression of VDR in intestinal mucosa.